A kind of molecular marker primer and its use and pcr method of 3v chromosome of tufted wheat

A technology of molecular markers and piloseum, applied in the field of molecular biology, can solve the problems of not being able to detect all regions of chromosomes, and achieve the effects of accelerating transfer and utilization, accelerating the breeding process, and improving efficiency

Inactive Publication Date: 2018-08-17
SAAS BIOTECH & NUCLEAR TECH RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Since the gene is generally a single copy on the chromosome, the primers designed based on the gene sequence can only detect the specific region where the gene is located on the chromosome, and cannot detect the entire region of the chromosome

Method used

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  • A kind of molecular marker primer and its use and pcr method of 3v chromosome of tufted wheat
  • A kind of molecular marker primer and its use and pcr method of 3v chromosome of tufted wheat
  • A kind of molecular marker primer and its use and pcr method of 3v chromosome of tufted wheat

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1. Synthesis of P. villosa 3V chromosome-specific SCAR marker primers

[0046] The 3V chromosome-specific SCAR marker primer TaGS5-C-1 of T. villosa consists of the following two primers: F: 5'-AGTTCCGAATCAAAACATAGTC-3', namely primer 1, as shown in the nucleotide sequence of SEQ ID NO.1 in the sequence table shown;

[0047] R: 5'-AAATCACAATCCTTCTTTATGC-3', that is, primer 2, as shown in the nucleotide sequence of SEQ ID NO.2 in the sequence table.

[0048] It can be synthesized according to conventional methods in the art, or synthesized by a primer synthesis company.

Embodiment 2

[0049] Embodiment 2, the PCR method of detecting the 3V chromosome of T. villosa

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Abstract

The invention provides a molecular marker primer for haynaldia villosa 3V chromosome, namely, a TaGS5-C-1 primer pair and an application of the primer in detecting the haynaldia villosa 3V chromosome. Besides, the invention also provides a PCR method for detecting the haynaldia villosa 3V chromosome on the basis of the primer.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a molecular marker primer and a PCR method for identifying the 3V chromosome of T. villosa. Background technique [0002] The annual diploid Triticum tufts (2n=14, VV) is an important relative of wheat, and its 1V-7V chromosomes carry multiple resistance genes (De Pace C 2011). Among them, chromosome 3V has genes for resistance to wheat take-all and eye spot disease (DePace C 2011). The common wheat Chinese spring (CS)-annual diploid Trichophyllum 3V addition line has excellent resistance to wheat stripe rust. In order to transfer the 3V chromosome of T. villosa to the genetic background of wheat, and further utilize the excellent resistance of the T. villosa 3V chromosome in the genetic improvement of wheat, the accurate identification and tracking of the T. villosa 3V chromosome has become an important task. At present, there have been many reports on the specific...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/6895
CPCC12Q1/6895C12Q2600/156
Inventor 张洁蒋云宣朴郭元林王颖
Owner SAAS BIOTECH & NUCLEAR TECH RES INST
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