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Method for increasing yield of cordycepin produced by cordyceps militaris liquid fermentation

A cordycepin, liquid fermentation technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of low yield of liquid culture, unable to meet market demand, etc., to achieve the biomass of Cordyceps militaris and Cordyceps militaris Effect of Cordycepin Production Ability Promotion

Inactive Publication Date: 2016-12-07
NEIJIANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the cultivation of Cordyceps militaris focuses on the research of solid culture, relatively little is involved in liquid culture, and the production of liquid culture involved is low, which cannot meet the market demand

Method used

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  • Method for increasing yield of cordycepin produced by cordyceps militaris liquid fermentation
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  • Method for increasing yield of cordycepin produced by cordyceps militaris liquid fermentation

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Embodiment 1

[0012] In the embodiment of the present invention, in order to obtain the optimal process for the preparation of Cordyceps militaris liquid primary species and the production of cordycepin by liquid fermentation, the Cordyceps militaris (L) link.NS-810 bacterial strain is used as the strain, and the inoculum amount ( spore concentration and the number of bacteria cakes), to explore the effects of different spore concentrations and bacteria cakes on the production effect of Cordyceps militaris liquid first-class species; through single single factor and orthogonal experiments, optimize the maximum yield of cordycepin produced by submerged fermentation of Cordyceps militaris Good medium. The specific description is as follows:

[0013] 1 Materials and methods

[0014] 1.1 Strain and culture medium

[0015] Cordyceps militaris [Cordyceps militaris(L)link.] was isolated from a fresh specimen of Cordyceps militaris collected in Wahuishan Nature Reserve, Jiulong County, Garze Tibe...

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Abstract

The invention discloses a method for increasing the yield of cordycepin produced by cordyceps militaris liquid fermentation. The method comprises the following steps: activation of culture, manufacturing of cordyceps militaris strains and cordyceps militaris culture; in the liquid strain manufacturing process, a mother strain manufactured by adopting the spore concentration of 3.0 x 108 CFU / mL or 4 to 5 strain blocks is used as first-stage seed liquid for enlarged cultivation of cordyceps militaris strains; in the cordyceps militaris culture process, a fermentation culture medium adopted contains 25 g / L of glucose, 100 g / L of potatoes, 18 g / L of fish peptone, 0.8 g / L of (NH4)2SO4, 1.0 g / L of KH2PO4, 0.5 g / L of MgSO4.7H2O, 5.0 g / L of silkworm chrysalis meal, 18 mg / L of VB1 and 1 L of water. According to the method, the biomass of cordyceps militaris is increased by 74.51 percent, and the total yield of cordycepin is increased by 238.52 percent. The method has an obvious promoting effect on the biomass of the cordyceps militaris and the capacity of cordyceps militaris producing the cordycepin.

Description

technical field [0001] The invention relates to the technical field of fungal fermentation, in particular to a method for increasing the yield of cordycepin produced by liquid fermentation of Cordyceps militaris. Background technique [0002] Cordyceps militaris is a model species of Ascomycota, Ergot, Ergotaceae and Cordyceps. The scientific name is Cordyceps militaris, also known as Cordyceps militaris, Cordyceps militaris, etc. It is also called Cordyceps militaris in some areas, and the number of natural resources distributed worldwide is very small. Cordycepin is the main active ingredient (especially nucleosides) in Cordyceps sinensis and Cordyceps militaris, and it is also the first nucleoside antibiotic isolated from fungi. Cordycepin is a natural source of medicine, which has the same functions as Cordyceps sinensis in traditional Chinese medicine, tonifying yin and yang and regulating the balance of the human body in two directions; it has better effects in protec...

Claims

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Application Information

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IPC IPC(8): C12P19/40C12R1/645
CPCC12P19/40
Inventor 张楠段辉国唐正义黎勇徐洁熊茂雷雨田
Owner NEIJIANG NORMAL UNIV