A kind of quality control method of total alkaloids of Sophora sophora
A quality control method and technology of total alkaloids, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of lack of reference substances, inability to objectively and reasonably control the quality of Chinese medicinal materials and their preparations, etc., to achieve easy popularization and mastery, High sensitivity and good stability
Active Publication Date: 2019-08-16
GUANGDONG MEDICAL UNIV
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[0005] The purpose of the present invention is to provide a quality control method for the total alkaloids of Sophora Sophora in view of the deficiencies in the prior art. Quality Control of Chinese Medicinal Materials and Their Preparations
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[0040] The present invention will be further illustrated in combination with the following examples.
[0041] The quality control method of the total alkaloids of Sophora sphaerocarpa in the present invention adopts a one-test and multiple evaluation method, and uses the sophoridine in the total alkaloids of Sophora sphaerocarpa as an internal reference material to detect matrine and gold in the total alkaloids of Sophora sphaerocarpa The quality of anthocyanine, sophocarpine, oxysophocarpine, matrine, oxymatrine, and Lymanine includes the following steps:
[0042] (1) Preparation of reference solution:
[0043] Precisely weigh the reference substances sophoridine, matrine, kininine, sophocarpine, oxysophocarpine, matrine, oxymatrine, and Lymanine, and place them in a 5ml volumetric flask. 0.05mol / LKH 2 PO 4 (Containing 2ml / L triethylamine) dissolve and dilute to the mark, and then respectively pass through a 0.22um filter membrane to obtain a concentration of 0.2914mg / ml sophoridin...
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The invention relates to the technical field of quality detection on traditional Chinese medicinal materials and particularly relates to a quality control method for total alkaloids of sophoraalopecuroides. According to the method, relative correction factors of sophoridine and other 7 kinds of alkaloids with traditional Chinese medicine activity are calculated by using a QAMS (quantitative analysis of multi-components by single marker) method in a manner of taking sophoridine of the total alkaloids of the sophoraalopecuroides as a substance for internal reference, and then, the content of the other 7 kinds of alkaloids in the total alkaloids of the sophoraalopecuroides is assayed. Compared with the prior art, the method provided by the invention is high in detection sensitivity and good in stability, the quality of the total alkaloids of the sophoraalopecuroides can be evaluated objectively, comprehensively and accurately, the problem that the quality of the traditional Chinese medicinal materials and preparations thereof cannot be objectively and reasonably controlled due to the deficiency of reference substances can be solved, and thus the method is of far reaching importance in quality control and clinical treatment effect guarantee.
Description
Technical field [0001] The invention relates to the technical field of quality detection of Chinese medicinal materials, in particular to a method for quality control of the total alkaloids of Sophora serrata. Background technique [0002] The difficulty of quality control and evaluation of traditional Chinese medicine lies in the complexity of multiple components of traditional Chinese medicine. It is difficult to characterize the quality of traditional Chinese medicine due to single component or index evaluation, and the method of multi-component quality control requires more reference materials. Due to the difficulty of separating the chemical reference substance of traditional Chinese medicine or the unstable monomer, difficult supply, high cost and other factors, there are many difficulties in the implementation of multi-component quality control. [0003] To this end, the researchers proposed a new mode of multi-index quality evaluation adapted to the characteristics of tradi...
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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 宋丽军赵文昌梁丽萍陈洪浪
Owner GUANGDONG MEDICAL UNIV