Method for detecting polysaccharide content of ganoderma extracts based on spectra

A technology of Ganoderma lucidum extract and polysaccharide content, applied in the direction of color/spectral characteristic measurement, etc., can solve problems such as reduction, pollution, and impact on the accuracy of results

Pending Publication Date: 2016-12-21
LIAONING INST OF SCI & TECH
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Problems solved by technology

Visible spectrophotometer is mostly used to measure the polysaccharide content of Ganoderma lucidum, and its analysis steps are quite cumbersome. The reduction of the analyzed index components...

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  • Method for detecting polysaccharide content of ganoderma extracts based on spectra
  • Method for detecting polysaccharide content of ganoderma extracts based on spectra
  • Method for detecting polysaccharide content of ganoderma extracts based on spectra

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[0037] In order to make the objects and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0038] The embodiment of the present invention provides a method for detecting polysaccharide content of Ganoderma lucidum extract based on spectrum, comprising the following steps:

[0039] S1. Select and set two spectroscopic instruments as the master and slave instruments respectively, and collect the spectra of the master and slave instruments of the Ganoderma lucidum extract sample;

[0040] S2. Perform multivariate scattering correction, Savitzky-Golay convolution smoothing, and MSC+S-G spectral preprocessing on the collected original spectrum in sequence, and then select and set a training set and a test set;

[0041] S3, generate the tw...

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Abstract

The invention discloses a method for detecting the polysaccharide content of ganoderma extracts based on spectra. Master-slave instrument spectra of samples of the ganoderma extracts are collected, spectra pretreatment of multiplicative scatter correction, Savitzky-Golay smoothing convolution and MSC+S-G is performed in sequence, and a two-dimensional correlation spectrum is generated; then multiscale decomposition is performed on the two-dimensional correlation spectrum by adopting an optimal two-dimensional wavelet basis, and all layers are respectively reconstructed; then the reconstructed spectrum of each layer is corrected by applying piecewise direct standardization, subsequently, prediction models which is based on partial least squares and is subjected to cross validation by a leave-one-out method are established, and finally, all of the prediction models are fused and evaluated according to calculated weight. The method has the advantages of fineness, precision and high efficiency, can be directly applied to the online detection of the polysaccharide content of the ganoderma extracts and has wide application and development prospects.

Description

technical field [0001] The invention relates to the technical field of food safety detection, in particular to a method for detecting the polysaccharide content of ganoderma lucidum extract based on spectrum. Background technique [0002] China is rich in edible fungi resources, and it is also one of the first countries to cultivate and utilize edible fungi. Edible mushroom extracts are rich in protein and amino acids, and their content is several to dozens of times that of ordinary vegetables and fruits. Edible fungi extract is also rich in various mineral elements: phosphorus, potassium, sodium, calcium, iron, zinc, magnesium, manganese, etc. and other trace elements. Ganoderma lucidum extract has high medicinal value, and has significant effects in improving human non-specific immune function, anti-oxidation, anti-tumor, anti-radiation, anti-aging, lowering blood sugar and blood fat. The active substances in Ganoderma lucidum extract are mostly polysaccharides and terpe...

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Application Information

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IPC IPC(8): G01N21/25
CPCG01N21/25
Inventor 楚冬海尚海巍贺凤伟
Owner LIAONING INST OF SCI & TECH
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