Identification method for effective components in ginkgo leaf preparation

Abstract

The invention discloses an identification method for the effective components in a ginkgo leaf preparation. The identification method comprises: (1) preparing a testing product: taking a proper amount of a ginkgo leaf preparation to be detected, adding n-butanol, extracting, filtering, evaporating the filtrate, and adding ethanol to the residue to dissolve so as to be adopted as the testing product solution; (2) preparing a reference substance solution: taking a proper amount of a ginkgo leaf reference preparation, and preparing the reference substance solution according to the step (1); and (3) carrying out thin layer chromatography: respectively sucking 1-10 [mu]lt of the testing product solution and the reference substance solution, respectively spotting on the same silica gel G plate, developing with an ethyl acetate, isopropyl alcohol, methanol and water mixture, taking out the thin plate, carrying out air drying, spraying a 1-5% aluminum chloride ethanol solution, placing in a 365 nm ultraviolet light lamp, viewing the fluorescence, and observing the spot. According to the present invention, with the identification method, the good separation effects of various effective components are good, the spots are clear, the Rf value is moderate, various effective components can be accurately identified, and the qualitative analysis and quality control are easily achieved.

Description

technical field [0001] The invention relates to the technical field of drug analysis, in particular to a method for identifying active ingredients in ginkgo leaf preparations, mainly through thin-layer chromatography to qualitatively analyze the active ingredients in ginkgo leaf preparations, especially flavonoids. Background technique [0002] Ginkgo biloba (Ginkgo Biloba) is the oldest existing seed plant in the world, and it is also the only survivor of the Ginkgo family dating back to the Permian period more than 200 million years ago. The herbal extract of Ginkgo biloba is used for drug treatment in China and many countries, and its medicinal value is very high. Various pharmacological activities such as peripheral blood circulation, lowering serum cholesterol and auxiliary anticancer. Flavonoid glycosides and terpenoid lactones in Ginkgo biloba are the main components that produce the above pharmacological activities, and they are also the main active ingredients and ...

AI Technical Summary

Problems solved by technology

The disadvantage of this method is that the boundaries between the spots are fuzzy, trailing, and line-like, indicating that the separation effect is poor, which is not conducive to identification, and it is difficult to carry out qualitative analysis and quality control of the active ingredients in the ginkgo biloba preparation.

The disadvantage of this method is that this method cannot separate the active ingredients in Ginkgo biloba extract, and the inspection results after spotting only show the main spots, which only indicate that the product contains Ginkgo biloba extract, and the degree of differentiation is poor.

The disadvantage of this method is that in the implementation case, only the text description and inspection results show that there are 8 yellow spots, and there is no atlas proof, so the accuracy of the identification cannot be judged

The weak point of this method is: as can be known from the developing agent screening result of embodiment 6, only when adopting stationary phase: 0.4% sodium acetate sodium carboxymethyl cellulose solution is the silica gel G plate of binding agent, developing agent: acetic acid When ethyl ester-butanone-methanol-water (5:3:1:1), the separation effect of total flavonol glycosides is clear, but there is a defect of edge effect

The disadvantage of this method is that due to the difference in the polarity of the substance, the choice of developing agent will make the concentration at the two edges of the thin-layer plate lower than that in the middle, resulting in inappropriate ratio shift, affecting the development and analysis results, and is not conducive to qualitative analysis. analyze

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