Equipment and method for High-throughput and rapid capturing and purifying of circulating tumor cells
A high-throughput technology for tumor cells, applied in the field of high-throughput rapid capture and purification of circulating tumor cells, can solve the problems of low automation, complicated operation, and low sensitivity, so as to improve automation, overcome low capture efficiency, and structural coherent compact effect
Inactive Publication Date: 2017-01-04
陈静
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Problems solved by technology
However, at present, this method is mostly manually operated in the laboratory, with low automation, low throughput, large errors, and easy to cause cross-contamination, especially easy to cause interference in subsequent molecular biology testing
In addition, long-term manual operation will also pose a potential threat to the health of the experimenter
[0010] To sum up, the current common methods for purifying circulating tumor cells in related technologies have complex operations, low degree of automation, low throughput and low sensitivity (depending on the characteristics of tumor cells, centrifugation time, temperature and other factors). ) and other technical issues
Method used
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[0072] The method for rapidly capturing circulating tumor cells provided by the present invention specifically includes the following steps:
[0073] 1. Filtration of blood samples (sample fluid):
[0074] a) Add 3ml of 4% paraformaldehyde solution to 2ml of peripheral blood sample, treat for 10 minutes, fix the cells in the peripheral blood, and inactivate possible pathogenic microorganisms at the same time.
[0075] b) adding to the assembled filter 22 at one time or in batches, the liquid level in the filter 2.2 should be lower than the bottom end of the overflow port 222 of the filter 22 after each addition;
[0076] c) Start the negative pressure device 25 and keep running it for 10-15 minutes, so that all blood samples pass through the filter membrane 23 under the action of negative pressure, so that circulating tumor cells can be trapped on the surface of the membrane by the microporous membrane.
[0077] In this step, since the diameter of circulating tumor cells is l...
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Abstract
The invention provides equipment and method for high-throughput and rapid capturing and purifying of circulating tumor cells, belonging to the technical field of biology. The equipment comprises a filtering module which comprises a filter, a filtering film and a negative pressure device, wherein the filtering film is arranged in the filter and is sealed with the inner wall of the filter; the negative pressure device is connected to one end of an outlet of the filter and is used for keeping the negative pressure in the filter; and the filtering film is used for intercepting the circulating tumor cells when the negative pressure device keeps the negative pressure in the filter. According to the equipment, sample liquid is input into the filter and the negatively device and can be automatically controlled, so that the process of intercepting the circulating tumor cells by virtue of the filtering film has the effects of high automation degree and throughput (which can be realized by increasing the number of filters) and the like.
Description
technical field [0001] The invention relates to the field of biotechnology, in particular to a device and method for rapidly capturing and purifying circulating tumor cells with high throughput. Background technique [0002] Circulating tumor cells (CTC, Circulating Tumor Cell) is a general term for various tumor cells existing in peripheral blood. In the process of tumor development, most of the CTCs are apoptotic or phagocytized after entering the peripheral blood due to spontaneous or therapeutic operation falling off from solid tumor lesions (primary lesions, metastases), and a few can escape and anchor and develop into metastases Foci, increase the risk of death in patients with malignant tumors, and play an important role in tumor invasion and metastasis. [0003] Circulating tumor cells shed into the circulation system, and many research results have confirmed their potential value in the diagnosis and prognosis of tumor metastasis, drug development, individualized t...
Claims
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IPC IPC(8): C12M1/36C12M1/12C12N5/09
CPCC12M41/48C12M45/04C12M47/04C12N5/0693C12N2509/10G01N2001/4088G01N1/40B01L2400/049B01L2300/0681A61M1/3482A61M1/3489G01N33/491
Inventor 陈静
Owner 陈静