High-throughput quick capturing and purifying apparatus and method for circulating tumor cells
Through the combination of negative pressure filter and automatic control system, the problem of low automation in the capture and purification process of circulating tumor cells is solved, and high-throughput and low cross-contamination capture of circulating tumor cells is achieved, which is suitable for large-scale screening detection and Personalized treatment.
Inactive Publication Date: 2017-03-22
SHANGHAI DIDA BIOTECH
View PDF6 Cites 12 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
However, at present, this method is mostly manually operated in the laboratory, with low automation, low throughput, large errors, and easy to cause cross-contamination, especially easy to cause interference in subsequent molecular biology testing
In addition, long-term manual operation will also pose a potential threat to the health of the experimenter
[0010] To sum up, the current common methods for purifying circulating tumor cells in related technologies have complex operations, low degree of automation, low throughput and low sensitivity (depending on the characteristics of tumor cells, centrifugation time, temperature and other factors). ) and other technical issues
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreExamples
Experimental program
Comparison scheme
Effect test
specific Embodiment
[0074] The method for rapidly capturing circulating tumor cells provided by the present invention specifically includes the following steps:
[0075] 1. Filtration of blood samples (sample fluid):
[0076] a) Add 3ml of 4% paraformaldehyde solution to 2ml of peripheral blood sample, treat for 10 minutes, fix the cells in the peripheral blood, and inactivate possible pathogenic microorganisms at the same time.
[0077] b) adding to the assembled filter 22 at one time or in batches, the liquid level in the filter 2.2 should be lower than the bottom end of the overflow port 222 of the filter 22 after each addition;
[0078] c) Start the negative pressure device 25 and keep running it for 10-15 minutes, so that all blood samples pass through the filter membrane 23 under the action of negative pressure, so that circulating tumor cells can be trapped on the surface of the membrane by the microporous membrane.
[0079] In this step, since the diameter of circulating tumor cells is l...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
| Property | Measurement | Unit |
|---|---|---|
| aperture size | aaaaa | aaaaa |
| rejection ratio | aaaaa | aaaaa |
| rejection ratio | aaaaa | aaaaa |
Login to View More
Abstract
The invention provides a high-throughput quick capturing and purifying apparatus and method for circulating tumor cells, and belongs to the field of biology. The apparatus comprises a filter module, the filter module comprises a filter, a filter membrane arranged in the filter and sealed to the inner wall of the filter, and a negative pressure device, the negative pressure device is connected to one outlet end of the filter and is used for keeping negative pressure in the filter, the filter membrane is used for intercepting circulating tumor cells when the negative pressure device keeps negative pressure in the filter, and carrying out cleaning after staining with a reagent automatically added via a reagent sampling module. It is possible to automatically control the delivery of sample liquid to both the filter and the negative pressure device, so that the process of filter membrane intercepting and circulating tumor cell staining is imparted high automation level, high throughput (that is achievable just by increasing the number of filters) and the like.
Description
technical field [0001] The invention relates to the field of biotechnology, in particular to a device and method for rapidly capturing and purifying circulating tumor cells with high throughput. Background technique [0002] Circulating tumor cells (CTC, Circulating Tumor Cell) is a general term for various tumor cells existing in peripheral blood. In the process of tumor development, most of the CTCs are apoptotic or phagocytized after entering the peripheral blood due to spontaneous or therapeutic operation falling off from solid tumor lesions (primary lesions, metastases), and a few can escape and anchor and develop into metastases Foci, increase the risk of death in patients with malignant tumors, and play an important role in tumor invasion and metastasis. [0003] Circulating tumor cells shed into the circulation system, and many research results have confirmed their potential value in the diagnosis and prognosis of tumor metastasis, drug development, individualized t...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/36C12M1/12C12N5/09
CPCC12M41/48C12M45/04C12M47/04C12N5/0693C12N2509/10G01N2001/4088G01N1/40B01L2400/049B01L2300/0681A61M1/3482A61M1/3489G01N33/491
Owner SHANGHAI DIDA BIOTECH