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A kind of theophylline-induced RNA interference switch system and its gene regulation method and application

A technology of RNA interference and switch system, which is applied in the field of theophylline-induced RNA interference switch system and its gene regulation, and can solve undeveloped problems

Active Publication Date: 2020-02-21
THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, theophylline-inducible "RNAi-ON" switch has not yet been developed

Method used

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  • A kind of theophylline-induced RNA interference switch system and its gene regulation method and application
  • A kind of theophylline-induced RNA interference switch system and its gene regulation method and application
  • A kind of theophylline-induced RNA interference switch system and its gene regulation method and application

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Embodiment Construction

[0022] The present invention will be further described in detail below through specific embodiments in conjunction with the accompanying drawings.

[0023] In the present invention, the so-called "RNA interference switch system" refers to a specially designed nucleotide sequence, and its structural characteristics are as follows: it includes an RNA interference off device, and the RNA interference off device itself is an artificial miRNA precursor or a Transcribed into an artificial miRNA precursor, the artificial miRNA precursor includes the miRNA precursor part and the theophylline RNA aptamer part fused to the loop region of the miRNA precursor part, and the theophylline RNA aptamer part can be bound by theophylline to hinder the occurrence of RNA interference.

[0024] Simultaneously, the RNA interference switching system of the present invention may also include an RNA interference opening device, the RNA interference opening device includes the above-mentioned RNA interf...

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Abstract

The invention discloses a theophylline-induced RNA disturbance on-off system and gene control method and use thereof. The theophylline-induced RNA disturbance on-off system at least comprises an RNA disturbance shutter. The RNA disturbance shutter is in itself an artificial miRNA fore-body or is transferred to an artificial miRNA fore-body. The artificial miRNA fore-body includes miRNA fore-body and the theophylline RNA aptamer of the annular section that is mixed with the miRNA fore-body; the theophylline RNA aptamer can be combined with theophylline to obstacle the interference effect of RNA. The RNA disturbance on-off system of the invention can activate or suppress RNA noise channel according to the theophylline concentration and dependence, thereby controlling the expression of target genes.

Description

technical field [0001] The invention relates to the technical field of gene regulation, in particular to a theophylline-induced RNA interference switch system and its gene regulation method and application. Background technique [0002] Nucleic acid switches, often defined as "functional non-coding RNA regions", control the expression of related genes by selectively binding metabolites, and appear on the transcriptional and post-transcriptional regulatory networks of various types of bacteria. A series of developments on nucleic acid switches have emerged, including gene therapy. Benefiting from advances in synthetic biology, these natural nucleic acid switches can be engineered into tools that recognize any given signal to regulate the expression of any given associated gene. [0003] The versatility of artificial nucleic acid switches expands the way of genetic research, among which theophylline aptamer is one of the most common and widely used devices for regulating gene...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/85A61K48/00A61K31/7088A61P35/00
CPCA61K31/7088C12N15/85C12N2800/107
Inventor 黄卫人陈志聪刘宇辰蔡志明
Owner THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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