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Nano-manganese dioxide for removing underground water heavy metal pollution and preparation method of nano-manganese dioxide for removing underground water heavy metal pollution

A technology of nano-manganese dioxide and heavy metals, applied in biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve problems such as pollution, complex processes, and high equipment requirements, so as to prolong service life and enhance oxidation and adsorption effect of ability

Active Publication Date: 2017-01-11
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main production methods of nano-manganese dioxide are: mechanical crushing method, electrochemical precipitation method, co-precipitation method, redox deposition method, self-assembly method, etc., but these methods have high requirements for equipment, complex process and easy to produce pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037]Inoculate the sterilized microbial growth medium with Leptothrixdiscophora SS-1 and culture at 25°C for 18 hours. Then the obtained mixture was subjected to high-speed centrifugation to obtain bacterial cells, and the precipitated bacterial cells were washed twice with sterile water. The obtained bacteria were inoculated into sterilized manganese oxide medium, in which the acid hydrolyzed casein was sterilized separately at 115°C at low temperature, and the glucose and HEPES were sterilized through a 0.22 μm filter membrane. After culturing at 25°C for 4 hours, add MnCl filtered through a 0.22 μm membrane 2 Make it to a concentration of 10mM and pass through the Na 3 VO 4 Make the concentration 5mM. Cultivate at 28°C for 18 hours to obtain a black precipitate which is the nano-manganese dioxide material.

[0038] 0.2g of the prepared manganese dioxide material was used to remediate 400ml of groundwater with an As(III) concentration of 1mg / L. By detecting and analyzi...

Embodiment 2

[0040] The mixed cells of Pseudomonas putida MnB1 and Bacillus sp SG-1 were inoculated into the sterilized microbial growth medium, and cultured at 30°C for 28 hours. Then the obtained mixture was subjected to high-speed centrifugation to obtain bacterial cells, and the precipitated bacterial cells were washed with sterile water for 3 times. The obtained bacteria were inoculated into sterilized manganese oxide medium, in which the acid hydrolyzed casein was sterilized separately at 115°C at low temperature, and the glucose and HEPES were sterilized through a 0.22 μm filter membrane. After culturing at 30°C for 8 hours, add MnCl filtered through a 0.22 μm membrane 2 Make its concentration 60mM and pass through 0.22μm filter Na 3 VO 4 The concentration was made 10 mM. Cultivate at 30°C for 24 hours to obtain a black precipitate which is the nano-manganese dioxide material.

[0041] 0.5g of the prepared manganese dioxide material was used to remediate 400ml of groundwater who...

Embodiment 3

[0043] Bacillus sp (Bacillus sp) SG-1 was inoculated into the sterilized microbial growth medium, and cultured at 28° C. for 22 hours. Then the obtained mixture was subjected to high-speed centrifugation to obtain bacterial cells, and the precipitated bacterial cells were washed with sterile water for 3 times. The obtained bacteria were inoculated into sterilized manganese oxide medium, in which the acid hydrolyzed casein was sterilized separately at 115°C at low temperature, and the glucose and HEPES were sterilized through a 0.22 μm filter membrane. After culturing at 28°C for 6 hours, add MnCl filtered through a 0.22 μm membrane 2 Make its concentration 40mM and pass through 0.22μm Na 3 VO 4 Make the concentration 8mM. Cultivate at 30°C for 22 hours to obtain a black precipitate which is the nano-manganese dioxide material.

[0044] 0.3g of the prepared manganese dioxide material was used to remediate 400ml of groundwater with a Pb(II) concentration of 40mg / L. After 20...

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Abstract

Disclosed are nano-manganese dioxide for removing underground water heavy metal pollution and a preparation method of the nano-manganese dioxide for removing underground water heavy metal pollution. The preparation method includes the steps of 1), inoculating one or more of Leptothrix discophora SS-1, Pseudomonas putida MnB1 and Bacillus sp SG-1 to a microbial growth medium and culturing at 25-30 DEG C; 2), centrifuging a mixture obtained in the step 1 to obtain a bacterial body and cleaning and precipitating the bacterial body; 3), inoculating the bacterial body obtained in the step 2 to a manganite culture medium and culturing at 25-30 DEG C; 4), adding MnCl2 and Na3VO4 into a product obtained in the step 3 and culturing at 28-30 DEG C so as to obtain black precipitate, namely the nano-manganese dioxide.

Description

technical field [0001] The invention belongs to the technical field of groundwater pollution restoration, and in particular relates to nano manganese dioxide for removing heavy metal pollution from groundwater. [0002] The present invention also relates to a preparation method of the above-mentioned nano manganese dioxide. Background technique [0003] Groundwater is a natural resource for human beings to survive. However, with the rapid development of modern industry and agriculture, heavy metal-containing pesticides and fertilizers are used in large quantities, making the pollution of heavy metals in groundwater increasingly serious. Heavy metals cannot be biodegraded, but are easy to accumulate in organisms, and eventually enter the human body through the food chain, causing various diseases and abnormalities in the body. In order to fundamentally prevent heavy metals from entering the environment and food chain, heavy metal pollution in groundwater must be remediated. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P3/00C12N1/20C12R1/40C12R1/07C12R1/01
CPCC12N1/20C12P3/00C12P39/00
Inventor 姜永海席北斗杨昱廉新颖梁冠男徐祥健董子萱
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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