Method for simultaneously detecting content of 4-ethyl phenol (4-EP) and 4-ethyl guaiacol (4-EG) in red wine

A technology of ethyl guaiacol and red wine, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of quality assurance

Inactive Publication Date: 2017-01-11
CHINA AGRI UNIV
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  • Method for simultaneously detecting content of 4-ethyl phenol (4-EP) and 4-ethyl guaiacol (4-EG) in red wine
  • Method for simultaneously detecting content of 4-ethyl phenol (4-EP) and 4-ethyl guaiacol (4-EG) in red wine
  • Method for simultaneously detecting content of 4-ethyl phenol (4-EP) and 4-ethyl guaiacol (4-EG) in red wine

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[0048] The present invention will be further described below by way of examples.

[0049] The present invention detects simultaneously the method for 4-ethylphenol and 4-ethylguaiacol content in red wine, and it comprises the following steps:

[0050] a. Preparation of internal standard: Accurately weigh 1mg of chromatographically pure p-cresol standard substance, dilute it with ethanol with chromatographically pure ethanol to a 1mL brown volumetric flask, store in a -20°C refrigerator, and use it as an internal standard use.

[0051] b. Headspace solid-phase microextraction: Add 10 mL of the red wine sample to be tested into a 15 mL headspace bottle equipped with a magnetic stirrer, add 100 μL of the internal standard prepared in step a and 2.5 g of NaCl. Place the headspace bottle on a magnetic heating and stirring device, pre-heat and balance at 50°C for 10 minutes, adjust the magnetic stirring speed to 850rpm, insert the extraction head, and perform headspace solid-phase ...

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Abstract

The invention belongs to the field of food detection, relates to a method for simultaneously detecting the content of 4-ethyl phenol (4-EP) and 4-ethyl guaiacol (4-EG) in red wine, and especially relates to a method for detecting through combination of headspace solid-phase micro-extraction-gas chromatography-mass spectrum. The invention aims to overcome the defect of the field of the present detection method for 4-EP and 4-EG in red wine and provides a method for simultaneous determination of the content of 4-EP and 4-EG in red wine by researching the optimal headspace solid-phase micro-extraction condition and the optimal gas chromatography-mass spectrum separation condition. The method mainly comprises the following steps: preparing an internal standard substance; performing headspace solid-phase micro-extraction; determining a standard curve; and performing gas chromatography-mass spectrum detection. The content of 4-EP and 4-EG in a sample can be calculated according to the obtained standard curve. Through verification, the method has the advantages of high speed, accuracy, convenience, higher precision and higher recovery rate.

Description

technical field [0001] The invention relates to the fields of food testing and food quality control, in particular to a method for determining the content of 4-ethylphenol and 4-ethylguaiacol in red wine, and in particular to a method for measuring the content of 4-ethylphenol and 4-ethylguaiacol by headspace solid-phase microextraction- Gas chromatography-mass spectrometry detection method. Background technique [0002] The smell of wine is an important indicator of wine quality and quality. A good smell will increase the complexity and coordination of wine and increase the purchase rate of consumers. Conversely, bad smells can cause wine defects. [0003] The smell of wine is composed of many substances. Among them, volatile phenols are a very important category, especially 4-ethylphenol (4-ethylphenol, 4-EP) and 4-ethylguaiacol (4-EG). [0004] 4-EP is often described as "hay shed" and "stable", while 4-EG is described as smoky and clove. At low concentration, 4-EP an...

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 战吉宬孙翔宇罗煊游义琳黄卫东
Owner CHINA AGRI UNIV
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