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Magnetic bead separation device and method based on microfluidics

A magnetic bead separation and microfluidic technology, which is applied in measurement devices, analytical materials, instruments, etc., can solve the problems of prone to errors and insufficient separation of magnetic beads, and achieves complete adsorption, convenient enrichment, and reduced errors. Effect

Inactive Publication Date: 2017-12-05
JIANGSU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method and system have the following disadvantages: first, artificially controlling the on-off of the electromagnetic coil, which is prone to errors; second, only one separation, resulting in insufficient separation of magnetic beads

Method used

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  • Magnetic bead separation device and method based on microfluidics
  • Magnetic bead separation device and method based on microfluidics
  • Magnetic bead separation device and method based on microfluidics

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Embodiment Construction

[0025] see figure 1 , The microfluidic-based magnetic bead separation device of the present invention includes a solid magnetic shielding material plate 2, the magnetic shielding material plate 2 is divided into upper and lower layers, both of which are cuboid solid magnetic shielding material plates. A group of micropipes from left to right are buried in the middle of the upper and lower layers. The main inlet pipe 14, the main outlet pipe 15 and the microfluidic chip 3 are all tightly embedded in the lower layer of the magnetic shielding material plate 2. The magnetic shielding material plate 2 The four corners of the upper and lower layers are fixed by four set screws 1, so that the upper and lower layers are tightly fixed together. The whole device has no gaps except micropipes, shielding all external magnetic interference.

[0026] The outer left side of the magnetic shielding material plate 2 is placed with three containers that respectively hold the immunomagnetic bead...

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Abstract

The invention discloses a microfluidic-based magnetic bead separation device and method in the field of microfluidic application. An immunomagnetic bead solution inlet pipe, a bacterial mixed solution inlet pipe and a sterile water inlet pipe are integrated into a main inlet pipe, a microfluidic chip is provided with an annular pipe connected to the main inlet pipe and a main outlet pipe, a micro-pump and a sixth solenoid valve are connected to the annular pipe in series, an annular colossal magnetoresistance chip set sleeves the annular pipe, the annular pipe is provided with a groove communicated with the interior of the annular pipe and integrated to the annular pipe, a right ahead side, a right astern side and an outlet side of the groove are provided with electromagnets, an outlet of the main outlet pipe is respectively connected to a mixed solution outlet pipe and a sterile water outlet pipe, through electromagnet and solenoid valve turning-on and off, magnetic beads are gathered, an immunization magnetic bead amount is detected through a magnetoresistance chip, the inner wall of the pipe is cleaned by sterile water and simultaneously, the immunomagnetic beads are collected.

Description

technical field [0001] The invention relates to the application field of microfluidics, in particular to a device for separating immunomagnetic beads with specific bacteria from an immunomagnetic bead solution and a mixed solution of bacteria. Immunomagnetic beads belong to immunological technology, which combines the unique advantages of solidified reagents with highly specific immunological reactions. Based on immunology, it penetrates into various fields such as pathology, physiology, pharmacology, microbiology, biochemistry, and molecular genetics. It is widely used in immunoassay, cell separation, biomacromolecule purification and molecular biology. Background technique [0002] At present, due to the advantages of fast speed, high concentration ratio, and simple equipment requirements, the immunomagnetic bead separation method has been widely used in practical applications. The existing immunomagnetic bead separation method generally consists of two steps: mixing and s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/543
CPCG01N33/54326
Inventor 张荣标周小溪
Owner JIANGSU UNIV
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