Interleukin-15 fusion protein for tumor targeting treatment

A fusion protein, tumor targeting technology, applied in the field of interleukin-15 fusion protein for tumor targeted therapy, can solve problems such as short half-life, achieve high-efficiency anti-tumor activity, improve anti-tumor efficacy, and overcome short half-life effect of the problem

Active Publication Date: 2017-02-08
BJ BIOSCIENCE INC
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Second, another deficiency of IL-15 is the short half-life

Method used

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  • Interleukin-15 fusion protein for tumor targeting treatment
  • Interleukin-15 fusion protein for tumor targeting treatment
  • Interleukin-15 fusion protein for tumor targeting treatment

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Embodiment Construction

[0033] fusion protein

[0034] In the present invention, "fusion protein", "PFC-1", "PFC-1 recombinant fusion protein" or "fusion molecule" are used interchangeably, and they refer to biologically active polypeptides, which are usually produced by recombinant, chemical or other suitable methods. Method Protein or peptide sequences covalently linked (ie fused) together. Fusion proteins may be fused to other peptide or protein sequences at one or more sites via a linker sequence. Alternatively, linker sequences can be used to assist in the construction of fusion molecules. Fusion proteins can exist in monomeric or multimeric (eg dimer) form.

[0035] In the present invention, "fusion protein" at least includes (i) IL-15 polypeptide, IL-15 polypeptide variant, or its functional fragment, (ii) IL-15Ra polypeptide, IL-15Ra polypeptide variant, or its functional Sexual fragments, (iii) Fc domain, Fc variants, or functional fragments thereof, and (iv) RGD polypeptides or variant...

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Abstract

The invention provides a tumor targeting fusion protein at least comprising (i) an IL-15 polypeptide, an IL-15 polypeptide variant, or a functional fragment thereof, (ii) an IL-15Ra polypeptide, an IL-15Ra polypeptide variant, or a functional fragment thereof, (iii) an Fc structural domain, an Fc variant, or a functional fragment thereof, and (iv) an RGD polypeptide or a variant thereof. The arraying sequence of the components of the fusion protein is preferably the RGD polypeptide-the Fc structural domain-the IL-15 polypeptide-the IL-15Ra polypeptide. On one hand, the tumor targeting fusion protein can enhance the anti-tumor efficacy of the IL-15, and on the other hand, the tumor targeting fusion protein overcomes the problem of short half-life of the IL-15, also can target to a tumor site, and targetedly acts on tumor cells. In addition, the tumor targeting protein can be efficiently expressed and purified, also has efficient antitumor activity, and has the potential to become an anti-tumor therapeutic drug.

Description

technical field [0001] The invention relates to the application of cytokine interleukin-15 in tumor targeting therapy, in particular to the antitumor effect of interleukin-15 fusion protein. Background technique [0002] Cytokines play an important role in regulating the human immune system, including anti-tumor immune responses. Some cytokines have been shown to have antitumor potential. Interleukin-15 (IL-15) has been extensively studied as a promising antitumor drug candidate. IL-15 and IL-2 share the β and γ chains of the receptor (IL-2 / 15βγ), but bind to different α receptor chains (IL-2Rα / IL-15Rα). The role of IL-15 is mainly through the trans-presentation mode, in which IL-15 binds to IL-15Rα expressed on the surface of antigen-presenting cells, then binds to IL-15βγ complexes on the surface of adjacent effector cells, and finally activates effector cells. Similar to IL-2, IL-15 can promote the proliferation of T cells and natural killer (NK) cells, promote the exp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K19/00C12N15/62A61K38/20A61K47/68A61K47/66A61P35/00
CPCA61K38/20C07K19/00C12N15/62C07K14/7155C07K2319/30C07K2319/70C07K14/5443A61P35/00A61K47/66A61K47/6813A61K38/2086C07K2319/00A61K38/08C07K7/06A61K38/1793A61P35/04C12N15/64C12N15/63C12N15/11
Inventor 李庆王忠
Owner BJ BIOSCIENCE INC
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