Multifunctional targeting drug delivery system with combined action of chemotherapy and phototherapy and preparation method thereof
A technology of targeted drug delivery and combined action, which is applied to medical preparations with non-active ingredients, medical preparations containing active ingredients, and pharmaceutical formulas, etc. Treatment effects and other issues, to achieve the effect of solving multidrug resistance, enhancing anti-tumor efficacy, and reducing toxic and side effects
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[0031] 1) Preparation of Ce6@PDA
[0032] Dissolve 20 mg PLGA and 2 mg Ce6 in 1 mL of acetone, and add them dropwise to 20 mL of deionized water under constant stirring in the dark, stir for 12 hours to remove acetone, centrifuge and wash with de-ethanol and deionized water to remove free Ce6, freeze-dry to obtain Ce6 / PLGA NP, disperse 50mgCe6 / PLGA NP in 50mL Tris-HCl buffer (pH8.5, 10mM), add 50mg dopamine hydrochloride, stir for 4 hours in the dark, then centrifuge the mixture and wash it three times with deionized water to remove untreated Polymerized dopamine was freeze-dried to obtain Ce6@PDA.
[0033] 2) HA-NH 2 Synthesis
[0034] Weigh 50mgHA, 145.6mgEDC.HCl, 88mgNHS, dissolve in 15mL formamide, activate the carboxyl group of HA under nitrogen protection, then slowly add it dropwise to ice-cold 10mL formamide solution containing 2.704mmol ethylenediamine, under nitrogen environment The reaction was stirred for 24 hours, and the reaction solution was precipitated in ex...
Embodiment 2
[0040] 1) Preparation of Ce6@PDA
[0041] Dissolve 30mgPLGA and 2mgCe6 in 1mL of acetone, and add it dropwise to 20mL of deionized water under constant stirring in the dark, stir for 12 hours to remove acetone, centrifuge and wash with de-ethanol and deionized water to remove free Ce6, freeze-dry to obtain Ce6 / PLGA NP, disperse 50mgCe6 / PLGA NP in 50mL Tris-HCl buffer (pH8.5, 10mM), add 50mg dopamine hydrochloride, stir for 6 hours in the dark, then centrifuge the mixture and wash it three times with deionized water to remove untreated Polymerized dopamine was freeze-dried to obtain Ce6@PDA.
[0042] 2) HA-NH 2 Synthesis
[0043] Weigh 50mgHA, 145.6mgEDC.HCl, 88mgNHS, dissolve in 15mL formamide, activate the carboxyl group of HA under nitrogen protection, then slowly add it dropwise to ice-cold 10mL formamide solution containing 2.704mmol ethylenediamine, under nitrogen environment The reaction was stirred for 24 hours, and the reaction solution was precipitated in excess c...
Embodiment 3
[0049] 1) Preparation of Ce6@PDA
[0050] Dissolve 30mgPLGA and 2mgCe6 in 1mL of acetone, and add it dropwise to 20mL of deionized water under constant stirring in the dark, stir for 12 hours to remove acetone, centrifuge and wash with de-ethanol and deionized water to remove free Ce6, freeze-dry to obtain Ce6 / PLGA NP, disperse 50mgCe6 / PLGA NP in 50mL Tris-HCl buffer (pH8.5, 10mM), add 50mg dopamine hydrochloride, stir in the dark for 8 hours, then centrifuge the mixture and wash it with ethanol and deionized water in sequence to The unpolymerized dopamine was removed, and Ce6@PDA was obtained by freeze-drying.
[0051] 2) HA-NH 2 Synthesis
[0052] Weigh 50mgHA, 145.6mgEDC.HCl, 88mgNHS, dissolve in 15mL formamide, activate the carboxyl group of HA under nitrogen protection, then slowly add it dropwise to ice-cold 10mL formamide solution containing 2.704mmol ethylenediamine, under nitrogen environment The reaction was stirred for 24 hours, and the reaction solution was pre...
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Description
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Application Information
- IPC
- A61K41/00; A61K31/704; A61K47/69; A61P35/00
- CPC
- A61K31/704; A61K41/0052; A61K41/0071; A61K47/6935; A61K47/6939; A61P35/00; A61K2300/00
- Inventors
- 许沛虎; 宁偲偲