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Method for distinguishing geographical indications of rice

A technology for geographical indications and rice, applied in the field of distinguishing geographical indications of rice, can solve the problems of unstable odor indicators, low discrimination precision, and great influence on results, and achieve stable mineral element fingerprint characteristics, fast analysis speed, and stable fingerprint characteristics. Effect

Inactive Publication Date: 2017-02-08
NANJING UNIV OF FINANCE & ECONOMICS +1
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Problems solved by technology

[0004] The patent with the publication number CN104914156A discloses a method for identifying the origin of rice, which is based on the detection of the mineral element content of the sample by a high-resolution inductively coupled plasma mass spectrometer, and establishes an origin discrimination model to achieve the purpose of origin identification; due to the introduction of There are fewer elements with significant differences, and the discrimination precision is lower
The patent of publication number CN104132968A discloses a method for identifying the origin of rice with geographical indications, which is based on the characteristic odor of rice samples collected by electronic noses, and establishes an origin discrimination model to achieve the purpose of origin identification; but the odor index is unstable, which has a great impact on the results

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  • Method for distinguishing geographical indications of rice
  • Method for distinguishing geographical indications of rice
  • Method for distinguishing geographical indications of rice

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: the determination of the pretreatment of sample and relevant element content

[0020] Samples were collected from farmers’ paddy fields in Sheyang County, Jiangsu Province, Wuchang City and Panjin City, Heilongjiang Province. Each sample was 1.0kg. A total of 54 rice samples were collected, including 19 samples in Sheyang County, Jiangsu Province, 15 samples in Wuchang City, Heilongjiang Province, and Panjin City. For 20 parts, the collected rice was hulled, milled, pulverized with a pulverizer for 30 seconds, and passed through a 250-mesh sieve for testing.

[0021] The 7700x ICP-MS produced by Agilent was used to detect the contents of the 24 kinds of mineral elements to be measured in the above rice samples from different origins. Weigh 0.5g of the above samples into the digestion tube respectively, add 7ml of high-grade pure concentrated nitric acid, after microwave digestion, use 2% nitric acid (high-grade pure) to dilute the digestion solution to a 1...

Embodiment 2

[0025] Example 2: Feasibility analysis of ICP-MS technology for rice origin identification

[0026] ICP-MS is used to characterize the fingerprint information of the 24 mineral elements in the sample. The amount of data information is large, and it is necessary to combine chemometric analysis methods to select effective information. The principle of principal component analysis is a statistical method that converts multiple interrelated numerical indicators into a few unrelated comprehensive indicators, that is, uses fewer indicators to replace and comprehensively reflect the original more information.

[0027] Use the software SPSS16.0 to conduct principal component analysis on the measured data, and perform PCA analysis based on the contents of the 24 mineral elements to be measured, such as figure 1 Shown is the loading diagram of the first three principal components. The first principal component mainly synthesizes B, Mg, Ti, V, Cr, Ga, Sr, Ba, Al, Mn, Fe, Ni, Zn, As, Sb, ...

Embodiment 3

[0028] Embodiment 3: stepwise discriminant analysis method establishes discriminant model

[0029] Discriminant analysis is to classify the researched object according to some observed indicators, obtain the so-called discriminant function, and then use the discriminant function to classify the samples of unknown classification.

[0030] From the results of principal component analysis, it can be seen that ICP-MS mineral element fingerprint analysis technology is feasible to identify the origin of rice. In order to further understand the results of mineral elements on the discrimination of rice origin, a step-by-step discriminant analysis was carried out on the mass concentrations of 24 mineral elements described in different types of samples, and variables that were effective for regional discrimination were screened out, unnecessary variables were eliminated, and a discriminant model was established. Cross-validation was performed to further confirm the reliability of the mo...

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Abstract

The invention discloses a method for distinguishing geographical indications of rice. On the basis of an ICP-MS (inductively coupled plasma mass spectrometry) process for simultaneously determining 24 mineral elements, stoichiometry is combined by using the mineral element contents as the indexes to establish a model for distinguishing the producing area of rice, thereby achieving the goal of distinguishing the producing area. The method is simple to operate, and has the advantages of stable mineral element fingerprint characteristics and multiple indexes for tracing detection, thereby enhancing the distinguishing precision.

Description

technical field [0001] The invention belongs to the technical field of agricultural product quality and safety analysis and detection, and in particular relates to a method for distinguishing geographical indications of rice. Background technique [0002] Geographical indication protected products refer to products that are planted or farmed or raw materials from the local area, and are produced and processed in the local area according to specific techniques. my country has implemented geographical indication product protection for thousands of geographical-origin products such as tea, grains, fruits, and medicinal materials, and more than 94% of geographical indication products involve agricultural products. Rice is an important food crop in the world. Rice quality is easily affected by special geographical factors such as local climate, soil, and water sources. In recent years, due to the increasingly serious environmental pollution and food safety problems, all kinds o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62G01N1/28
CPCG01N27/62G01N1/286
Inventor 方勇杜梦佳胡秋辉毛波吴亚君
Owner NANJING UNIV OF FINANCE & ECONOMICS
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