Microbial fertilizer for restoring acidic soil and preparation method thereof
A technology for microbial fertilizer and acid soil, applied in the field of microbial fertilizer for remediation of acid soil and its preparation, can solve the problems of reducing the degree of nutrient absorption of crops, neglecting the improvement of microbial activity in acid soil, etc., so as to improve crop yield, increase microbial activity, The effect of adaptability
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Embodiment 1
[0033] The preparation method of the acid soil conditioner of the present embodiment may further comprise the steps:
[0034] (1) Microbial agent preparation: Take 5g of glucose, 5g of beef extract, 10g of peptone, 5g of NaCl and 1L of distilled water and mix well, adjust the pH value to 7.0, and sterilize to obtain the primary medium; After being respectively transferred to the primary medium prepared above, they were cultured separately for 48 hours at a temperature of 37°C and a rotational speed of 250r / min; Then, the fermented liquid was obtained by expanding the culture at a temperature of 37°C and a rotating speed of 250r / min for 48 hours; then the fermented liquid of each bacteria was mixed in proportion, and the bran after sterilizing and cooling was mixed according to its sum The mass ratio of the mixed bacteria is 30:1, mixed evenly, air-dried naturally, sealed in bags, and made into bacterial agents. Among them, Desulfovibrio desulfuricans, Bacillus subtilis, Bacil...
Embodiment 2
[0037] The preparation method of the acidic soil conditioner of the present embodiment may further comprise the steps:
[0038] (1) Microbial agent preparation: Take 5g of glucose, 5g of beef extract, 10g of peptone, 5g of NaCl and 1L of distilled water and mix well, adjust the pH value to 7.0, and sterilize to obtain the primary medium; After being transferred to the primary medium prepared above, they were cultured separately for 48 hours at a temperature of 37°C and a rotational speed of 250r / min; the obtained strains were mixed according to the weight ratio of wet bacteria, and then added to the Amplify in the obtained primary culture medium, expand and cultivate for 48 hours at a temperature of 37°C and a rotation speed of 250r / min to obtain a fermentation broth; The bran is mixed evenly according to the mass ratio of 30:1 to the mixed bacteria, air-dried naturally, bagged and sealed to make a bacterial agent. The sulfate-reducing bacteria (Desulfovibrio desulfuricans), ...
Embodiment 3
[0041] The preparation method of the acidic soil conditioner of the present embodiment may further comprise the steps:
[0042](1) Microbial agent preparation: Take 5g of glucose, 5g of beef extract, 10g of peptone, 5g of NaCl and 1L of distilled water and mix well, adjust the pH value to 7.0, and sterilize to obtain the primary medium; After being transferred to the primary medium prepared above, they were cultured separately for 48 hours at a temperature of 37°C and a rotational speed of 250r / min; after mixing the obtained strains according to the weight ratio of wet bacteria, they were added to the prepared Amplify in the obtained primary culture medium, expand and cultivate for 48 hours at a temperature of 37°C and a rotation speed of 250r / min to obtain a fermentation broth; The bran is mixed evenly according to the mass ratio of 30:1 to the mixed bacteria, air-dried naturally, bagged and sealed to make a bacterial agent. The sulfate-reducing bacteria (Desulfovibrio desul...
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