Nitrobacterium continuous production method and production equipment thereof

A technology of nitrifying bacteria and production methods, applied in the direction of sterilization methods, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of cumbersome process, unfavorable long-term storage, and impurity of ammonia oxidizing bacteria, so as to prevent random The effects of effluent loss, increased bacteria attachment rate, and simple storage method

Active Publication Date: 2017-02-22
郭洪伟 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN102250783B has invented a method for the enrichment and cultivation of ammonia oxidizing bacteria, which uses activated sludge as an inoculation source and landfill leachate as a medium for intermittent cultivation. The main disadvantage of this invention is that the enriched ammonia oxidizing bacteria are impure and have The reported final ammonia oxidation efficiency has only increased by 20%, and this process belongs to batch fermentation, so the product takes a long time and has low efficiency
CN102757913A describes a method for the production of high-activity nitrifying bacteria products. Although this method overcomes the problem of the interaction between the medium when adding the medium, the activity described in the patent does not have specific data to explain the level of activity. , and the process of making the final product is relatively cumbersome and requires hot air circulation and drying, which not only requires additional costs, but also has a great impact on the activity of ammonia nitrogen bacteria
CN101709278A has invented a large-scale cultivation method of high-concentration nitrosating bacteria. This method uses a coagulant for flocculation treatment. The flocculant will reduce the activity of nitrosating bacteria and nitrifying bacteria, which is not conducive to long-term preservation
CN102757913A Use an adsorption device to carry out adsorption and enrichment of nitrifying bacteria. The bacteria after each batch of enrichment need to be continuously desorbed, and the process is relatively cumbersome

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  • Nitrobacterium continuous production method and production equipment thereof
  • Nitrobacterium continuous production method and production equipment thereof
  • Nitrobacterium continuous production method and production equipment thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] NB1 commercially available nitrifying bacteria culture process

[0044] NB1 cultivation process: press figure 1 Install all the devices and pipelines, and after commissioning, first add tap water to the culture device, when it reaches about 30% of the effective volume, add NM1 medium and nitrifying bacteria NB1, turn on the aeration, and control the pH at 7.50 through NM3 ~7.80, the temperature is maintained at 30°C, and the membrane module does not open at this stage. When the ammonia nitrogen drops to 57mg / L, the membrane filter module is opened, and only the filtrate is discharged, so that the filtrate is continuously discharged at a speed of 55.54L / h (1333L / d). Start the NM-4 feed pump, add NM-4 at a rate of 2.08L / h (50L / d), and start the water pump at the same time to keep the total volume of the culture system at 1000L. When Rn reaches 10mgN / L / h (measure the filtrate ammonia nitrogen in the effluent after every 24h≦57mg / L), use NM-2 instead of NM-4 for feeding, ...

Embodiment 2

[0046] NB2 commercially available nitrifying bacteria culture process

[0047] NB2 cultivation process: press figure 1 Install all the devices and pipelines and complete the debugging.

[0048] First, tap water is added to the cultivation device. When it reaches about 30% of the effective volume, NM1 medium and nitrifying bacteria NB2 are added, aeration is started, and the pH is controlled at 7.50-7.80 by NM3, and the temperature is maintained at 30°C. At this stage, the membrane Component does not turn on. When the ammonia nitrogen drops to 57mg / L, the membrane filter module is opened, and only the filtrate is discharged, so that the filtrate is continuously discharged at a speed of 55.54L / h (1333L / d). Start the NM-4 feed pump, add NM-4 at a rate of 2.08L / h (50L / d), and start the water pump at the same time to keep the total volume of the culture system at 1000L. When Rn reaches 10mgN / L / h (measure the filtrate ammonia nitrogen in the effluent after every 24h≦57mg / L), use ...

Embodiment 3

[0050] NB3 commercially available nitrifying bacteria culture process

[0051] NB3 cultivation process: press figure 1 Install all the devices and pipelines and complete the debugging.

[0052] First, tap water is added to the cultivation device. When it reaches about 30% of the effective volume, NM1 medium and nitrifying bacteria NB3 are added, aeration is turned on, and the pH is controlled at 7.50 to 7.80 by NM3, and the temperature is maintained at 30°C. At this stage, the membrane Component does not turn on. When the ammonia nitrogen drops to 57mg / L, the membrane filter module is opened, and only the filtrate is discharged, so that the filtrate is continuously discharged at a speed of 55.54L / h (1333L / d). Start the NM-4 feed pump, add NM-4 at a rate of 2.08L / h (50L / d), and start the water pump at the same time to keep the total volume of the culture system at 1000L. When Rn reaches 10mgN / L / h (measure the filtrate ammonia nitrogen in the effluent after every 24h≦57mg / L),...

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Abstract

The invention discloses a nitrobacterium continuous production method and production equipment thereof. The production equipment comprises a culture tank, a nutrient solution conveying system, an oxygen conveying system, a water inlet pipe, an alkalinity adjusting alkali supplementing tank, a concentrated supplementing tank without activated carbon, a make-up pump, a drainage pump, a bacterium liquid drainage pump and a membrane assembly in the culture tank. The nutrient solution conveying system comprises a nutrient solution storage tank, a nutrient solution pump and a nutrient solution conveying pipe, and the nutrient solution storage tank is connected with the nutrient solution pump through the nutrient solution conveying pipe. The oxygen conveying system comprises a fan and an inverted-umbrella-shaped aeration head, an output pipe of the fan is introduced from the lower portion of the culture tank to be connected with the inverted-umbrella-shaped aeration head, and the output end of the nutrient solution conveying pipe, an output pipe of the concentrated supplementing tank without the activated carbon, the water inlet pipe and a drainage pipe of the alkalinity adjusting alkali supplementing tank are mounted above the culture tank. The activity of the obtained product has quantitative indexes, and finally the product reaches 500 mgN/Lbacterium liquid/h. Thalli and a nutrient solution can be separated more efficiently.

Description

technical field [0001] The invention relates to the field of nitrifying bacteria production, in particular to a method for continuous production of nitrifying bacteria and production equipment thereof. Background technique [0002] In the natural nitrogen cycle, nitrifying bacteria play an irreplaceable role. Nitrifying bacteria are a type of nitrogen that can convert ammonia nitrogen into nitrate nitrogen and then finally convert it into nitrogen gas and return to the atmosphere through the action of denitrifying bacteria. The nitrification process is completed by two types of bacteria. Nitrite oxidizing bacteria oxidize ammonia nitrogen into nitrite nitrogen. The main bacterial species are Nitrosomonas, Nitrosospira, Nitrosovibrio and Nitrosococcus; nitrate oxidizing bacteria oxidize nitrite nitrogen to nitrate nitrogen, the main species are Nitrospira, Nitrobacter, nitrifying Nitrococcus and Nitrospina etc. The above bacteria are Gram-negative bacteria, and all are chem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12M1/12C12M1/04C12R1/01
CPCC12M25/00C12M29/04C12M29/06C12M41/26C12N1/20
Inventor 张岩
Owner 郭洪伟
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