Primer for PCR (polymerase chain reaction) diagnosis of wild porcine parvovirus infection, kit with primer for PCR diagnosis of wild porcine parvovirus infection and application of primer and kit

Abstract

The invention discloses a primer for PCR (polymerase chain reaction) diagnosis of wild porcine parvovirus infection, a kit with the primer for PCR diagnosis of wild porcine parvovirus infection and application of the primer and the kit. The primer for PCR diagnosis of wild porcine parvovirus infection is as shown in SEQ ID NO:1 and SEQ ID NO:2. The primer can be used for preparing the kit for PCR diagnosis of wild porcine parvovirus infection, and the kit comprises a forward primer, a reverse primer, a negative control, a positive control, 10*PCR buffer solution, Taq enzyme and dNTP. The primer and the kit with the primer are available for specific distinguishing of porcine parvovirus vaccine immunity and wild porcine parvovirus infection and are high in sensitivity and repeatability and real and reliable in detection result. Utilization of special instruments in a detection process is avoided, and simplicity and convenience in detection, short detection time and simplicity in result determination are realized.

Description

technical field [0001] The invention relates to the technical field of molecular diagnosis of animal pathogens, in particular to a primer for PCR diagnosis of porcine parvovirus wild virus infection, a kit containing the primer and an application thereof. Background technique [0002] Porcine parvovirus disease is a pig reproductive disorder caused by porcine parvovirus (Porcine Parvovirus, PPV). It is an infectious disease characterized by infection of embryos and fetuses to death, mummies, deformed fetuses and normal fetuses. Porcine parvovirus disease is widespread and occurs worldwide. The disease can occur throughout the year, but occurs frequently during the sows' farrowing and mating season from April to October each year. The disease is endemic or sporadic. After the occurrence of the disease, the pig farms may continue to experience reproductive failure for several years. The main target of the disease is young sows, especially first-born sows, which are highly in...

AI Technical Summary

Problems solved by technology

The specificity of the primers has a great influence on the reaction. If the specificity of the primers is not high, non-target bands may be generated during the amplification process, which will affect the determination of the detection results.

At present, there have been research reports on PCR detection methods of PPV at home and abroad. These methods require high technical content or have not been commercialized. They can only be used in professional laboratories and are not suitable for large-scale clinical diagnosis and field application.

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