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Asparagus cochinchinensis tissue culture seedling culture medium capable of improving asparagine

A technology of asparagine and asparagine group, applied in the direction of plant regeneration, application, organic fertilizer, etc., to achieve the effect of shortening disinfection time, reducing production cost and simple operation

Active Publication Date: 2017-03-08
贵州仙草农业集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The invention provides an asparagine tissue culture medium for raising asparagine, so as to solve the problems of how to increase the content of asparagine, the main active ingredient of asparagine, in the existing tissue culture medium, and improve economic benefits, etc.

Method used

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  • Asparagus cochinchinensis tissue culture seedling culture medium capable of improving asparagine

Examples

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Effect test

preparation example Construction

[0082] The preparation method of the asparagine tissue culture medium for improving asparagine comprises the following steps:

[0083] S1: After peeling the lemons, crush the fruit with a blender, and pass the obtained juice through a 3000-5000 mesh sieve to obtain fruit juice;

[0084] S2: The fruit juice, potassium salt, phosphorus salt, nitrogen salt, calcium salt, magnesium salt, iodine salt, boron salt, molybdenum salt, zinc salt, manganese salt, copper salt, iron salt, vitamin, amino acid obtained in step S1 , indole butyric acid, gibberellin, 6-benzyl adenine, naphthalene acetic acid, kinetin, co-solvent, dispersant, compatibilizer, antibacterial agent, water mixed, the microwave power is 95-135W, the temperature is Stir at 64-70°C for 28-52min at a rotational speed of 200-400r / min to prepare the mixed solution A;

[0085] S3: Add a conditioner and a stabilizer to the mixed solution A prepared in step S2, and stir for 14-24 minutes at a temperature of 47-53° C. and a r...

Embodiment 1

[0088] A medium for asparagus tissue culture seedlings for increasing asparagine, comprising the following raw materials in parts by weight: 250 parts of fruit juice, 3 parts of potassium salt, 2.5 parts of phosphorus salt, 1.8 parts of nitrogen salt, 0.9 part of calcium salt, 0.8 parts of magnesium salt, 0.4 part of iodine salt, 0.3 part of boron salt, 0.2 part of molybdenum salt, 0.3 part of zinc salt, 0.2 part of manganese salt, 0.1 part of copper salt, 0.1 part of iron salt, 0.13 part of vitamin, 0.14 part of amino acid, indole 0.08 parts of butyric acid, 0.08 parts of gibberellin, 0.07 parts of 6-benzyl adenine, 0.08 parts of naphthalene acetic acid, 0.05 parts of kinetin, 0.15 parts of conditioner, 2.2 parts of co-solvent, 1.8 parts of dispersant, 1.5 parts of compatibilizer 1.2 parts of stabilizer, 2.2 parts of antibacterial agent, 3000 parts of water;

[0089] The fruit juice is lemon juice;

[0090] The potassium salt is potassium nitrate;

[0091] The phosphorus sa...

Embodiment 2

[0116] A culture medium for asparagus tissue culture seedlings for increasing asparagine, comprising the following raw materials in parts by weight: 122 parts of fruit juice, 2.3 parts of potassium salt, 1.4 parts of phosphorus salt, 1.2 parts of nitrogen salt, 0.7 part of calcium salt, 0.6 parts of magnesium salt, 0.3 part of iodine salt, 0.2 part of boron salt, 0.2 part of molybdenum salt, 0.3 part of zinc salt, 0.1 part of manganese salt, 0.1 part of copper salt, 0.1 part of iron salt, 0.09 part of vitamin, 0.08 part of amino acid, indole 0.05 parts of butyric acid, 0.06 parts of gibberellin, 0.04 parts of 6-benzyl adenine, 0.06 parts of naphthaleneacetic acid, 0.03 parts of kinetin, 0.17 parts of conditioning agent, 1.4 parts of co-solvent, 1.2 parts of dispersing agent, and 1 part of compatibilizer parts, 0.9 parts of stabilizer, 1.5 parts of antibacterial agent, 2500 parts of water;

[0117] The fruit juice is lemon juice;

[0118] The potassium salt is potassium nitrat...

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Abstract

The invention discloses an asparagus cochinchinensis tissue culture seedling culture medium capable of improving asparagine. The culture medium comprises the following raw materials: water juice, potassium salt, phosphorus salt, nitrogen salt, calcium salt, magnesium salt, iodized salt, boron salt, molybdenum salt, zinc salt, manganese salt, copper salt, iron salt, vitamin, amino acid, indolebutyric acid, gibberellin, 6-benzyl adenine, naphthylacetic acid, an agonist, a conditioner, a cosolvent, a dispersing agent, a compatilizer, a stabilizer, an antibacterial agent and water. The quality of seedlings obtained by the asparagus cochinchinensis tissue culture seedling culture medium is good, the survival rate is high and the effective component asparagines is high.

Description

technical field [0001] The invention belongs to the technical field of asparagus planting, and in particular relates to a culture medium of asparagus tissue culture seedlings for increasing asparagine. Background technique [0002] Asparagus is a commonly used traditional Chinese medicine, which has the effects of nourishing yin and promoting body fluid, moistening the lungs and clearing the heart. In recent years, the demand for wild asparagus in the market has increased significantly, resulting in the destruction of wild asparagus, so the artificial cultivation technology of asparagus has gradually developed. Since most of the asparagus currently on the market are scattered and planted by farmers, and the varieties are relatively miscellaneous, it is difficult to guarantee the content of asparagine, the active ingredient of asparagus. Contents of the invention [0003] The invention provides a culture medium of asparagine tissue culture seedlings with increased asparagi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C05G3/00
CPCA01H4/001C05B7/00C05G3/00C05C3/00C05C5/02C05C11/00C05D1/00C05D3/00C05D5/00C05D9/00C05D9/02C05F11/00C05F11/02
Inventor 韦波
Owner 贵州仙草农业集团有限公司
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