Triple peptide Cec Md3js and preparation method and application thereof

A triple peptide and antimicrobial peptide technology, applied in the field of genetic engineering, can solve the problems of cumbersome separation and purification steps, and achieve the effects of good thermal stability, low hemolysis, and simple separation and purification steps

Pending Publication Date: 2017-03-08
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0004] Cecropins (cecropins) is currently the most clearly studied and most effective natural antimicrobial peptide; the homology between Musca domestica cecropin (Cec Md) and cecropins is 81%, but Musca domestica cecropins The peptide still has a certain hemolytic activity, and the separation and purification steps are cumbersome

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  • Triple peptide Cec Md3js and preparation method and application thereof
  • Triple peptide Cec Md3js and preparation method and application thereof
  • Triple peptide Cec Md3js and preparation method and application thereof

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Embodiment Construction

[0032] The present invention will be described in detail below with reference to the drawings and specific embodiments, but it should be understood that the protection scope of the present invention is not limited by the specific embodiments.

[0033] The present invention provides a triple peptide Cec Md3js, the nucleotide sequence for encoding the triple peptide Cec Md3js is shown in SEQ ID NO.1, and the amino acid sequence of the triple peptide Cec Md3js is shown in SEQ ID NO.2 Show.

[0034] Based on the same inventive concept, the present invention also provides a method for preparing the triple peptide Cec Md3js, comprising the following steps:

[0035] Step 1, synthesizing the target gene whose nucleotide sequence is shown in SEQ ID NO.3.

[0036] It should be noted that, in order to increase the expression of antimicrobial peptides, a tandem design was adopted, following the principle of Pichia pastoris preference, and the nucleotide sequence for encoding the triparti...

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Abstract

The invention discloses a triple peptide Cec Md3js. A nucleotide sequence for encoding the triple peptide Cec Md3js is shown as SEQ ID NO. 1, and an amino acid sequence of the triple peptide Cec Md3js is shown as SEQ ID NO. 2. An antibacterial peptide CecMd prepared by using the triple peptide Cec Md3js has antibacterial activity and high thermal stability on various bacteria, is easy to separate, purify and operate, and is suitable for large-scale industrial production. The triple peptide Cec Md3js has good application prospects in the fields of antibacterial drugs, food antibacterial agents, feed additives and the like. Moreover, the invention further provides a preparation method of the triple peptide Cec Md3js.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a triple peptide Cec Md3js, a preparation method and an application. Background technique [0002] Antimicrobial peptides are an effective barrier for organisms to resist the invasion of external pathogenic microorganisms. They have broad-spectrum antibacterial effects and are not prone to drug resistance. They have good application prospects in agriculture, medical care, food, animal feed and other fields. At present, thousands of antimicrobial peptides have been discovered, but the types of antimicrobial peptides that can be applied clinically are still limited. Therefore, people hope to obtain novel antimicrobial peptides with better activity through molecular design and reengineering. [0003] Since antimicrobial peptides are small molecular peptides, their cloning, expression, separation, purification and detection procedures are relatively cumbersom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435C07K1/36C07K1/34C07K1/16C07K1/12C12N15/12C12N15/81A61K38/17A61P31/04A23K20/195
CPCA61K38/00C07K14/43577Y02A50/30
Inventor 姜宁张爱忠祁丽张晨雪张宇李天阳
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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