Testing method of influence of polymethyl methacrylate on spinal metastasis tumor cell

A technology of polymethyl methacrylate and testing methods, applied in biological testing, biochemical equipment and methods, microbial determination/inspection, etc.
CN106480182AInactive Publication Date: 2017-03-08THE FIRST AFFILIATED HOSPITAL OF CHONGQING MEDICAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
THE FIRST AFFILIATED HOSPITAL OF CHONGQING MEDICAL UNIVERSITY
Publication Date
2017-03-08
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a testing method of influence of polymethyl methacrylate on spinal metastasis tumor cell. The testing method includes steps of treating a primary culture spinal metastasis tumor for 24 h by PMMA bone cement at different stages; measuring temperatures of inner part and surface of the bone cement, and respectively observing three different polymerization stages which are polymerization period, wire-drawing period, cooling and condensing period; particularly observing the influence of heat released by the bone cement in the wire drawing period on the tumor cell proliferation and apoptosis; measuring the cell apoptosis and cell cycle by a flow cytometry; measuring relationship among PMMA and Bcl-2, Bax, Caspase 3, Caspase 8, Fas and PARP in cell apoptosis; cyclin D1. The invention learns about the molecular mechanism of the in-vitro PMMA for the primary culture spinal metastasis tumor, and provides a solid theoretical basis for treating spine metastatic tumors by percutaneous centrum injected bone cement.
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Description

technical field

[0001] The invention belongs to the technical field of spinal metastatic tumors, in particular to a method for testing the influence of polymethyl methacrylate on spinal metastatic tumor cells. Background technique

[0002] Currently, the consequences of spinal metastases include pathological fractures, spinal cord compression, anemia, and hypercalcemia. Lung, breast, renal cell carcinoma, lymphoma, thyroid and prostate cancers tend to metastasize to the spine. Among them, breast cancer is particularly prone to metastasize to the spine, and it is found that it accounts for about 2 / 3 of bone metastases. About one-third of these spinal lesions are symptomatic, causing severe pain, neurological damage, mechanical instability, and ultimately disability and a severe deterioration in quality of life. However, the treatment of symptomatic spinal metastases still chooses palliative therapy. The goals of treatment include restoring and protecting neurological functi...

Claims

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