Testing method of influence of polymethyl methacrylate on spinal metastasis tumor cell

A technology of polymethyl methacrylate and testing methods, applied in biological testing, biochemical equipment and methods, microbial determination/inspection, etc.

Inactive Publication Date: 2017-03-08
THE FIRST AFFILIATED HOSPITAL OF CHONGQING MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Indications for surgery in patients with spinal metastases are

Method used

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  • Testing method of influence of polymethyl methacrylate on spinal metastasis tumor cell
  • Testing method of influence of polymethyl methacrylate on spinal metastasis tumor cell
  • Testing method of influence of polymethyl methacrylate on spinal metastasis tumor cell

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Embodiment Construction

[0050] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0051] The application principle of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0052] Such as figure 1 Shown, the test method that the polymethyl methacrylate of the embodiment of the present invention influences spinal metastases cell comprises the following steps:

[0053] S101: Treat primary cultured vertebral metastatic tumor cells with PMMA in vitro, using Bcl-2, Bax, Caspase 3, Caspase 8, Fas and other apoptotic genes as entry points;

[0054]S102: Use realtime-PCR, Western blot, and flow cytometry to detect changes in tumor cell apoptosis rate and possib...

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Abstract

The invention discloses a testing method of influence of polymethyl methacrylate on spinal metastasis tumor cell. The testing method includes steps of treating a primary culture spinal metastasis tumor for 24 h by PMMA bone cement at different stages; measuring temperatures of inner part and surface of the bone cement, and respectively observing three different polymerization stages which are polymerization period, wire-drawing period, cooling and condensing period; particularly observing the influence of heat released by the bone cement in the wire drawing period on the tumor cell proliferation and apoptosis; measuring the cell apoptosis and cell cycle by a flow cytometry; measuring relationship among PMMA and Bcl-2, Bax, Caspase 3, Caspase 8, Fas and PARP in cell apoptosis; cyclin D1. The invention learns about the molecular mechanism of the in-vitro PMMA for the primary culture spinal metastasis tumor, and provides a solid theoretical basis for treating spine metastatic tumors by percutaneous centrum injected bone cement.

Description

technical field [0001] The invention belongs to the technical field of spinal metastatic tumors, in particular to a method for testing the influence of polymethyl methacrylate on spinal metastatic tumor cells. Background technique [0002] Currently, the consequences of spinal metastases include pathological fractures, spinal cord compression, anemia, and hypercalcemia. Lung, breast, renal cell carcinoma, lymphoma, thyroid and prostate cancers tend to metastasize to the spine. Among them, breast cancer is particularly prone to metastasize to the spine, and it is found that it accounts for about 2 / 3 of bone metastases. About one-third of these spinal lesions are symptomatic, causing severe pain, neurological damage, mechanical instability, and ultimately disability and a severe deterioration in quality of life. However, the treatment of symptomatic spinal metastases still chooses palliative therapy. The goals of treatment include restoring and protecting neurological functi...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/18G01N33/68G01N33/574
CPCC12Q1/6886C12Q2600/158G01N33/5011G01N33/57407G01N33/68G01N2333/4739
Inventor 胡侦明赵军郝杰沈皆亮
Owner THE FIRST AFFILIATED HOSPITAL OF CHONGQING MEDICAL UNIVERSITY
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