Phellinus igniarius strain rejuvenating method

A technology of Phellinus linteus and strains, applied in the fields of botanical equipment and methods, horticulture, plant cultivation, etc., can solve the problem of slow growth of mycelium, and achieve the effects of high mushroom formation rate, fast growth and strong resistance.

Inactive Publication Date: 2017-03-15
ZHENJIANG AGRI SCI & TECH IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the production, the purification and rejuvenation of Phellinus fungi are generally carried out by separating the young edge part of the fruit body and the original base for purification and culture. This method is easy to operate, but the growth of the isolated fungus hyphae is relatively slow. Basically, it takes 10-12 days to fully grow

Method used

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  • Phellinus igniarius strain rejuvenating method

Examples

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Comparison scheme
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Embodiment 1

[0022] The present embodiment illustrates the method for mycelia separation in the culture medium of the present invention:

[0023] The operation steps are as follows:

[0024] (1) In the Phellinus cultivation shed, select a single flower with typical fruiting bodies, pure color, positive mushroom shape, and no pests and diseases to collect Phellinus fungus with a fungus cultivation bag, and there is a culture material in the cultivation bag.

[0025] (2) Wipe the surface of the cultivation bag with 3-5% potassium permanganate solution.

[0026] (3) Move the cultivation bag into the inoculation box or ultra-clean workbench, and use a sterilized scalpel or a single blade to remove the 0.5 cm square bag film in the middle and lower part of the bag.

[0027] (4) Use a sterilized inoculation needle to remove the old hyphae on the surface, pick a medium with mycelium the size of rice grains in the culture material, and connect it to the center of the inclined test tube through th...

Embodiment 2

[0030] This example illustrates the superiority of the bacterial strains isolated by the method of Example 1 compared to the bacterial strains obtained by the conventional fruiting body tissue separation method.

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Abstract

The invention relates to a phellinus igniarius strain rejuvenating method. The phellinus igniarius strain rejuvenating method comprises the following steps: selecting a single ganoderma cultivation bag which has the characteristics of typical sporocarp, pure chromaticness, regular mushroom shape and freeness from pest and disease damage in a phellinus igniarius cultivation tent by an intra-compost hypha separation method; wiping the surface of the cultivation bag by a 3 to 5 percent potassium permanganate solution; transferring the cultivation bag into an inoculation hood or an ultra-clean workbench, and removing a bag membrane of 0.5cm<3> from the lower-middle part of the bag with a sterilized dissecting scalpel or a single blade; removing aged hypha from the surface with a disinfected inoculation needle, picking a culture medium carrying hypha of a rice size from the compost, and inoculating an aseptic area of alcohol burner flame into the center of a beveled glass tube; and lastly, cultivating at a dark place at the temperature of 25 DEG C for 2 to 3 days, selecting a test tube without pollution and with robust hypha, picking newly-born hypha under an aseptic condition and inoculating the newly-born hypha to a new beveled culture medium to cultivate the obtained pure mycelium. The phellinus igniarius strain rejuvenating method has the advantages of high hypha growth speed, high resistance, high mushroom mature rate and high yield.

Description

technical field [0001] The invention relates to the rejuvenation of degenerated edible fungus strains, in particular to a method for rejuvenation of Phellinus fungus strains. Background technique [0002] Sanghuang, the name of a traditional Chinese medicine, see "Dictionary of Chinese Medicine". It belongs to Fungi, Basigiomycota, and Hymenochaxtales in classification. It is a hard porous fungus and is a famous medicinal fungus in China, Japan, Korea and other places. Modern studies have confirmed that Phellinus can improve the body's immunity and reduce the side effects of anticancer agents, so it can be used to assist radiotherapy and chemotherapy for tumor patients. In addition, Phellinus is also effective for women's irregular menstruation and other gynecological diseases. [0003] The breakthrough in the key technology of artificial cultivation of Phellinus chinensis in my country has enabled the realization of large-scale artificial cultivation of Phellinus chinensis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
CPCA01G18/00
Inventor 吴琴燕庄义庆陈宏洲
Owner ZHENJIANG AGRI SCI & TECH IND
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