Agriophyllum squarrosum callus and induced proliferation method thereof

A technology of callus, sand rice, applied in horticultural methods, botanical equipment and methods, horticulture, etc., to achieve the effect of high induction rate

Active Publication Date: 2017-03-15
NORTHWEST INST OF ECO-ENVIRONMENT & RESOURCES CAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The purpose of the present invention is to provide a method for inducing the proliferation of Sami...

Method used

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  • Agriophyllum squarrosum callus and induced proliferation method thereof
  • Agriophyllum squarrosum callus and induced proliferation method thereof
  • Agriophyllum squarrosum callus and induced proliferation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] (1) Select healthy Shami seeds, place Shami seeds in a triangular flask, add 3% sodium hypochlorite solution, seal the mouth of the bottle with a breathable bottle stopper, place on a shaker, shake and rinse for 15 minutes, and place in running water Rinse for 1 hour, then wash with sterile water for 2 to 3 times, inoculate the seeds on 1 / 2MS medium for cultivation, and obtain sterile seedlings of Shami after 12 days. The temperature of the incubation room is 25°C, and the intensity of ordinary fluorescent light.

[0047] Cut the stems of aseptic seedlings of Shami seedlings with a seedling age of 10 days, and inoculate them in induction medium to induce Shami callus culture. The induction medium is MS medium supplemented with 0.2mg / L 6- Composition of BA, 2.0mg / L 2,4-D, 0.70% agar and 2.0% sucrose, pH 5.9. The induction time is 30 days, and the induction results are as follows figure 1 Shown by figure 1 It can be seen that this method successfully induced callus.

[0048]...

Embodiment 2

[0050] Embodiment 2 is a change on the basis of Embodiment 1. The content mentioned in Embodiment 1 will not be described in detail in this embodiment.

[0051] The difference between Example 2 and Example 1 is that the induction medium in Example 2 is: MS medium supplemented with 0.3mg / L 6-BA, 3.0mg / L 2,4-D, 0.70% agar and It is composed of 2.0% sucrose and has a pH of 5.9. The remaining steps are the same as in Example 1.

Embodiment 3

[0053] Embodiment 3 is a change on the basis of embodiment 1. The content mentioned in embodiment 1 will not be described in detail in this embodiment.

[0054] The difference between Example 3 and Example 1 is that the induction medium in Example 3 is: MS medium supplemented with 0.5 mg / L 6-BA, 4.0 mg / L 2,4-D, 0.70% agar and It is composed of 2.0% sucrose and has a pH of 5.9. The remaining steps are the same as in Example 1.

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Abstract

The invention provides an agriophyllum squarrosum callus and an induced proliferation method thereof, and relates to the technical field of plant cell culture. The method uses an MS culture medium as the culture medium with the addition of 6-BA with the concentration of 0.2-0.5 mg/L, 2, 4-D with the concentration of 2.0-4.0 mg/L, 0.70% agar and 2.0% sucrose. The pH value is 5.8-6.0. The proliferation culture medium is the MS culture medium with the addition of 6-BA with the concentration of of 0.1 mg/L, 2, 4-D with the concentration of 1.0 mg/L, 0.70% agar and 2.0% sucrose. The method can use the agriophyllum squarrosum roots, stems and leaves as the explants to successfully induce callus with high inductivity. Moreover, the agriophyllum squarrosum callus prepared according to the method is also provided to compensate for the deficiency of the prior art.

Description

Technical field [0001] The invention relates to the technical field of plant cell culture, in particular to a kind of Shami callus and a method for inducing proliferation thereof. Background technique [0002] Shami, also known as Shapeng and Dengxiangzi, is an annual herbaceous plant of Chenopodiaceae. It is widely distributed in the desert areas of Northwest, North and Northeast China, as well as parts of Henan, Hebei, Shanxi and Tibet, Mongolia and the former Soviet Union. Siberia and Central Asia are also distributed. Shami has the basic characteristics of cold tolerance, cold tolerance, barren tolerance, wind erosion resistance to sand burial, and sandy soil. It is commonly found in mobile sand dunes or semi-mobile sand dunes and riverbank sand banks. It is a common sand plant in northern my country and a pioneer town plant in fixed mobile sand dunes. [0003] Shami can play the role of windbreak and sand fixation; its seeds are rich in nutrients and are a good functional fo...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 赵昕张继伟陈国雄
Owner NORTHWEST INST OF ECO-ENVIRONMENT & RESOURCES CAS
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