A strain of Gluconacetobacter high-yielding phenyllactic acid and its preparation method for phenyllactic acid

Abstract

The invention discloses a high-yield phenyllactic acid acetic acid bacterium and a method for preparing phenyllactic acid from its fermented liquid. Gluconacetobacter provided by the invention ( Gluconacetobacter sp.) strain FBFS 97 was deposited in the China Center for Type Culture Collection on July 11, 2016, with the preservation number CCTCC NO:M 2016388. When the Gluconacetobacter FBFS 97 provided by the present invention was fermented for 13 days in a liquid medium without adding phenylalanine at 30°C and 160 r / min, the content of phenyllactic acid in the fermentation broth was 95.71 mg / L; adding 1 mg / mL phenylalanine, fermented under the same conditions for 17 days, the content of phenyllactic acid in the fermentation broth was 412.05 mg / L. No relevant research has been reported.

Description

technical field [0001] The invention belongs to the field of food biotechnology, and relates to a strain of Gluconacetobacter sp. FBFS 97 with high yield of natural food preservative phenyllactic acid and a preparation method thereof. Background technique [0002] Food preservatives refer to food additives that can prevent food spoilage caused by microorganisms and prolong the shelf life of food. According to the production method, it can be divided into two categories: synthetic and natural. Among them, synthetic food preservatives have low cost and good antiseptic effect, but their safety is controversial. With the improvement of people's living standards and the improvement of food safety requirements , the research and development of natural food preservatives with higher safety has become a research hotspot. Natural preservatives derived from microorganisms are an important source of natural food preservatives. At present, natural preservatives derived from microorgan...

AI Technical Summary

Problems solved by technology

Studies have shown that Geotrichum candidum, lactic acid bacteria, and propionic acid bacteria can convert phenylalanine and phenylpyruvate to produce phenyllactic acid, but the conversion rate of phenylalanine is low, and although the conversion rate of phenylpyruvate is high, its physical and chemical properties Instability and high cost make it difficult to realize industrial production of phenyllactic acid by biotransformation

[0005] The present invention isolates an acetic acid bacterium, Gluconacetobacter sp. FBFS 97, which produces high-yield natural food preservative phenyllactic acid from the soil, and provides a method for preparing phenyllactic acid from the bacterium by fermentation, so as to facilitate the industrialization of phenyllactic acid in the future The microbial screening of production has expanded the scope, and related research has not been reported

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