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Polypeptide probe for specific identification of lysophosphatidic acid, preparation and applications thereof

A lysophosphatidic acid, specific technology, applied in the directions of preparations, peptides, fluorescence/phosphorescence, etc. for in vivo tests, can solve problems such as difficulty in meeting early diagnosis

Inactive Publication Date: 2017-03-22
EAST CHINA UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the ELISA method and the FAT method are limited by their sensitivity and specificity, whether it is detecting antigens or antibodies, it is difficult to meet the needs of early diagnosis

Method used

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  • Polypeptide probe for specific identification of lysophosphatidic acid, preparation and applications thereof
  • Polypeptide probe for specific identification of lysophosphatidic acid, preparation and applications thereof
  • Polypeptide probe for specific identification of lysophosphatidic acid, preparation and applications thereof

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preparation Embodiment 1

[0084] The preparation of the polypeptide probe 1 specifically recognizing LPA comprises the following steps:

[0085] (1) Put Rink amide resin (309mg, 0.810mmol / g, 0.25mmol, 1equiv.) and Fmoc-Lys(Fmoc)-OH (0.75mmol, 3.0equiv) in the reaction flask, add organic base (2.5mmol, 10equiv) and a condensing agent (0.75mmol, 3.0equiv), in DMF solution, reacted at room temperature for 3 hours, then removed the Fmoc protecting group to obtain a resin coupled with lysine;

[0086] (2) Repeat step (1) successively, wherein the raw amino acid is replaced by Fmoc arginine (1.5mmol, 6.0equiv), Fmoc glycine (1.5mmol, 6.0equiv), Fmoc aspartic acid ( 1.5mmol, 6.0equiv), Fmoc lysine (1.5mmol, 6.0equiv), and finally obtain a resin sequentially coupled with the above amino acids;

[0087] (3) Resin and Fmoc-Aeg-G (Boc) that step (2) is sequentially coupled with the above-mentioned amino acid 2 -OH (1.5mmol, 6.0equiv) was placed in a reaction flask, an organic base (5.0mmo, 10equiv) and a conden...

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Abstract

The present invention provides an early-stage ovarian cancer serum marker lysophosphatidic acid (LPA), a polypeptide probe for specific recognition of the LPA, and preparation and applications of the polypeptide probe, wherein the polypeptide probe is formed by linking a polypeptide Peptide, a linking group linker, and a fluorescent group Dye through amide bonds. According to the present invention, the polypeptide probe can accurately detect the presence of the LPA in the clinical serum sample at the early stage of ovarian cancer, and the polypeptide probe can be combined with an near infrared imager to image so as to be used for the early diagnosis of ovarian cancer, such that the polypeptide probe specifically recognizes the LPA based on the high sensitivity and the high specificity of the LPA in the early diagnosis of ovarian cancer so as to provide the important clinical diagnosis basis for the early diagnosis of ovarian cancer; and the probe has characteristics of simple use operation and low cost, and provides the good application prospect for cancer biomarker detection, near infrared fluorescence imaging, and other fields.

Description

technical field [0001] The present invention relates to the field of medicines for tumor diagnosis, and in particular to the preparation and application of the ovarian cancer early serum marker lysophosphatidic acid (LPA) and its specific recognition polypeptide probe and polypeptide probe. The LPA polypeptide probe is The novel polypeptide probe detects LPA content by specifically binding to LPA in cells, serum or living body, and can be further used to detect ovarian cancer. Background technique [0002] Ovarian malignancy (ovarian cancer) has been described as one of the deadliest cancers due to its insidious onset. The early symptoms of ovarian cancer are not obvious, and the initial symptoms are only abdominal pain or distension, and gastrointestinal indigestion, which have not attracted enough attention. In 2004 alone, approximately 24,000 women in the United States developed ovarian cancer, and 14,000 women died from ovarian cancer. Worldwide, there are an estimated...

Claims

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Application Information

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IPC IPC(8): C07K5/09C07K5/093C07K7/06C07K7/08G01N21/64A61K49/00
Inventor 吴君臣姚德帆林之张文旭赖燕华李尚田禾
Owner EAST CHINA UNIV OF SCI & TECH
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