Preparation method of a fluorescent polarized fluorescent magnetic molecular imprinting sensor

A technology of magnetic molecular imprinting and fluorescence polarization, which is applied in the fields of fluorescence/phosphorescence, chemical instruments and methods, and material analysis through optical means, which can solve the problem of only detecting analytes with fluorescent properties and reduce the cost of preparation , high detection sensitivity and wide application range

Active Publication Date: 2019-01-18
XIAN TECH UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method overcomes the problem that traditional fluorescent molecular imprinted sensors can only detect analytes with fluorescent properties

Method used

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  • Preparation method of a fluorescent polarized fluorescent magnetic molecular imprinting sensor
  • Preparation method of a fluorescent polarized fluorescent magnetic molecular imprinting sensor
  • Preparation method of a fluorescent polarized fluorescent magnetic molecular imprinting sensor

Examples

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preparation example Construction

[0030] Step one, Fe 3 o 4 Preparation of nanoparticles

[0031] 1.72gFeCl 2 • 4H 2 O and 4.72gFeCl 3 •6H 2 O and 80mL of deionized water were added to the flask, and nitrogen was continuously introduced into the flask and placed in a DF-101S collector type constant temperature heating magnetic stirrer and stirred vigorously to dissolve it; when the temperature rose to 80°C, 10ml of ammonia The aqueous solution was slowly dripped into the flask and reacted for 30 minutes; the product was separated under the action of an external magnetic field; washed 6 times with deionized water to remove unreacted substances, dried and stored;

[0032] Step two, Fe 3 o 4 @SiO 2 preparation of

[0033] 300mg of magnetic nanoparticles, 50mL of isopropanol and 4mL of ultrapure water were sonicated for 15-20mim, and then 5mL of ammonia water and 2mL of tetraethylorthosilicate were added in sequence; the reaction was continuously stirred at room temperature for 12h; the product was collec...

Embodiment 1

[0042] A method for preparing a fluorescent polarization fluorescent magnetic molecular imprinting sensor, the specific steps are:

[0043] Step one, Fe 3 o 4 Preparation of nanoparticles

[0044] 1.72gFeCl 2 • 4H 2 O and 4.72gFeCl 3 •6H 2 O and 80mL of deionized water were added to the flask, and nitrogen was continuously introduced into the flask and placed in a DF-101S collector type constant temperature heating magnetic stirrer and stirred vigorously to dissolve it; when the temperature rose to 80°C, 10ml of ammonia The aqueous solution was slowly dripped into the flask and reacted for 30 minutes; the product was separated under the action of an external magnetic field; washed 6 times with deionized water to remove unreacted substances, dried and stored;

[0045] Step two, Fe 3 o 4 @SiO 2 preparation of

[0046] 300mg of magnetic nanoparticles, 50mL of isopropanol and 4mL of ultrapure water were sonicated for 20mim, and then 5mL of ammonia and 2mL of tetraethylor...

Embodiment 2

[0054] A method for preparing a fluorescent polarization fluorescent magnetic molecular imprinting sensor, the specific steps are:

[0055] Step one, Fe 3 o 4 Preparation of nanoparticles

[0056] 1.72gFeCl 2 • 4H 2 O and 4.72gFeCl 3 •6H 2 O and 80mL of deionized water were added to the flask, and nitrogen was continuously introduced into the flask and placed in a DF-101S collector type constant temperature heating magnetic stirrer and stirred vigorously to dissolve it; when the temperature rose to 80°C, 10ml of ammonia The aqueous solution was slowly dripped into the flask and reacted for 30 minutes; the product was separated under the action of an external magnetic field; washed 6 times with deionized water to remove unreacted substances, dried and stored;

[0057] Step two, Fe 3 o 4 @SiO 2 preparation of

[0058] 300mg of magnetic nanoparticles, 50mL of isopropanol and 4mL of ultrapure water were sonicated for 15mim, and then 5mL of ammonia and 2mL of tetraethyl o...

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Abstract

The invention relates to a sensor sensitive element, and a preparation method thereof, and more specifically relates to a preparation method of a fluorescence polarization fluorescent magnetic molecular imprinted sensor. The preparation method comprises following steps: 1, Fe3O4 nano particles are prepared; 2, Fe3O4@SiO2 is prepared; 3, fluorescent Fe3O4@SiO2 is prepared; 4, fluorescent MIP-Fe3O4@SiO2 is prepared; 5, non-imprinting fluorescent magnetic polymer (NIP-Fe3O4@SiO2) is prepared. According to the preparation method, a molecularly imprinted polymer with both fluorescence and magnetic performance is prepared, fluorescence polarization technique is adopted to detect the molecularly imprinted polymer and a substance to be detected, and the fluorescence polarization fluorescent magnetic molecular imprinted sensor is obtained. The preparation method is capable of solving a problem of detection of conventional fluorescent molecular imprinted sensors that only substances with fluorescence can be detected, simplifying preparation technical process, increasing detection sensitivity, and reducing the limit of detection to ng / L.

Description

technical field [0001] The invention relates to a sensor sensitive element and a preparation method thereof, in particular to a preparation method of a fluorescent polarization fluorescent magnetic molecular imprint sensor. Background technique [0002] Biosensors have become a very important topic in the field of analytical chemistry and biochemistry due to their high sensitivity and specificity. However, due to the inherent defects of biomolecules (high environmental requirements, difficult to preserve for a long time, and biomolecules are derived from living organisms, preparation and purification are cumbersome and expensive), the development of biosensors is limited. Obtaining cheap and stable recognition elements is one of the keys to the further development of biosensors. In the past two decades, a new method of synthesizing "artificial antibodies", that is, molecular imprinting technology, has been studied. In particular, the research on surface molecular imprintin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F292/00C08F220/06C08F220/56C08F8/34C08F285/00C08F222/14C08J9/26C09K11/06C09K11/02G01N21/64
CPCC08F8/34C08F285/00C08F292/00C08J9/26C08J2351/10C09K11/025C09K11/06C09K2211/1088G01N21/6445C08F220/06C08F222/102
Inventor 赵晨贾光锋陆文总李滚孟祥艳潘海仙任郁苗
Owner XIAN TECH UNIV
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