A continuous preparation method of antibody (or antigen) coated microspheres
A technology of microspheres and antibodies, applied in the preparation of microspheres, microcapsule preparations, instruments, etc., can solve the problem of no antibody reported in the literature, and achieve the effects of controllable product quality, mild reaction conditions and good contact.
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Embodiment 1
[0040] refer to figure 1 The industrial process of the present invention follows the following steps: (1) First, the Buffer A solution of the microspheres in S1 and the Buffer A solution of the condensing agent pass through the P1 and P2 metering pumps, and are mixed into the mixing module M1 according to a certain ratio ; (2) After the reaction in R1, it passes through the filter module F1 to remove impurities, (3) Then the buffer B1 is pumped into the module M2 through the metering pump, and mixed with the activated microspheres, (4) The B1 solution of the antibody is passed through The metering pump enters the mixing module M3 to mix with the activated microspheres, and then reacts in the reaction module R2, (5) then passes through the filtration module F2 to remove excess antibodies and impurities, (6) the effluent is mixed with the buffer in the mixing module M4 B2 is mixed, and the mixed solution is finally collected in the collection module C. This example describes a...
Embodiment 2
[0042] refer to figure 2 The industrial process of the present invention follows the following steps: (1) First, the Buffer A solution of the microspheres in S1 and the Buffer A solution of the antibody (or antigen) pass through the P1 and P2 metering pumps, and are injected into the mixing module according to a certain ratio M1 is mixed; (2) After the reaction in R1, it passes through the filter module F1 to remove impurities, (3) The effluent is mixed with buffer B2 in the mixing module M4, and the mixed solution is finally collected in the collection module C. This example describes the general physisorption method for coating microspheres.
Embodiment 3
[0044] (1) The device used: refer tofigure 1 Determine the connection mode of the microchannel reactor, the number of mixed reaction modules is determined according to the flow rate and reaction residence time, and the heat exchange medium is ethanol.
[0045] (2) In a microchannel reactor with a diameter of 200 µm, a buffer solution (15 mg / mL, flow rate 1 mL / min) of polystyrene microspheres (200 nm) with carboxyl groups on the surface and a buffer solvent of condensing agent EDC (5 mg / mL mL, flow rate 1mL / min) mixed in the mixing unit M1 (temperature 25 degrees), stay in R1 for 300 seconds (temperature 25 degrees), and then pass into the filter unit F1 (temperature 25 degrees), the residence time is 120 seconds, and then in the module M2 was mixed with buffer B1 (1 mL / min). Afterwards, the B1 solution of NGAL antibody (5mg / mL, 1mL / min) was pumped into the mixing module M3 through the metering pump to mix with the activated microspheres, and then stayed in the reaction module...
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