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Method for rapidly propagating potato microtubers by using potato stem segments

A potato and micro-potato technology is applied in the fields of botanical equipment and methods, application, root crop cultivation, etc., can solve the problems of high reproduction coefficient, time-consuming and laborious, complicated operation, etc., and achieves high reproduction coefficient, convenient transportation and simple operation. Effect

Active Publication Date: 2019-11-05
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has low cost and high reproduction coefficient, but this technology has the following defects: First, it needs to use N-dimethylaminosuccinic acid and KH 2 PO 4 to induce axillary buds
Second, the isolated axillary buds need to be treated with exogenous hormones before they can be cut. The operation is complicated and time-consuming.

Method used

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  • Method for rapidly propagating potato microtubers by using potato stem segments
  • Method for rapidly propagating potato microtubers by using potato stem segments
  • Method for rapidly propagating potato microtubers by using potato stem segments

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: Utilize the method of potato stem section cuttage technology to propagate color potato powder variety ' cherie ' miniature tuber rapidly, carry out the following steps successively:

[0032] 1), the preparation of cultivation medium:

[0033] Mix peat, vermiculite, and perlite in a mass ratio of 1:1:1 to prepare a cultivation substrate;

[0034] 2), transplanting of test-tube seedlings:

[0035] In early April, divide the cultivation medium into seedling pots and water thoroughly. Take the virus-free potato test-tube seedlings with good growth and plant height of 7-8cm from the tissue culture room, plant them in seedling pots after seedling training, put the seedling pots in a net shed covered with sunshade nets for cultivation, and use 40 mesh The insect-proof nets cover the seedling pots, and water them in good time according to conventional techniques. The daytime temperature is 28-30°C, the nighttime temperature is 18-20°C, and the humidity is 70%-9...

Embodiment 2

[0048] Embodiment 2. The potato variety in Embodiment 1 was changed from "cherie" miniature potato to "Zhongshu No. 3"; the rest were identical to Embodiment 1.

[0049] The result is:

[0050] Step 3): When the potato plant grows to about 30cm, it takes about 10 days; the multiplication coefficient is 9-10 times.

[0051] Step 5): about 18 days, the leaf nodes of the cut potato stem section produced about 1g of miniature potatoes;

[0052] Step 7): About 7 days, the harvested potato microtubers grow into seedlings; it takes about 9 days for the seedlings to grow to about 30 cm; the multiplication factor is 9 to 10 times.

Embodiment 3

[0053] Embodiment 3. The potato variety in Embodiment 1 is changed from "cherie" miniature potato to "Chuan 56"; the rest are equal to Embodiment 1.

[0054] The result is:

[0055] Step 3): When the potato plant grows to about 30cm, it takes about 9 days; the multiplication coefficient is 7-8 times.

[0056] Step 5): about 19 days, the leaf nodes of the cut potato stem section bear about 1g of miniature potatoes;

[0057] Step 7): About 7 days, the harvested potato microtubers grow into seedlings; it takes about 10 days for the seedlings to grow to about 30 cm; the multiplication factor is 7-8 times.

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Abstract

The invention discloses a method for rapidly propagating a mini potato by using a potato stem segment. The method comprises the following steps: a cultivation substrate is prepared; the cultivation substrate is loaded into a nursery pot, and after thorough watering, a 7 to 8 cm tall potato seedling is transplanted into the nursery pot; then the potato seedling is put in a net shed with a shade net and an insect net cover for cultivation; when the cultivated potato plant grows to 28 to 32 cm tall, the top of the potato plant is cut off, and then a stem segment of the potato plant is cut into a stem segment with a compound leaf; the cultivation substrate is loaded into an aperture disk and is thoroughly watered; the potato plant stem segment is placed in the aperture disk for cultivation with the morphological upper end heading upwards, and the petiole base is just covered by the cultivated substrate during cutting; after a mini potato with the weight between 0.8 and 1.2 g grows at a leaf node of the potato stem segment cutting, the stem segment is pulled out, and the mini potato is harvested.

Description

technical field [0001] The invention relates to a rapid propagation technology of potato microtubers, in particular to rapid propagation of potato microtubers by using the cutting technology of potato stem segments. Background technique [0002] Potato (Solanum tuberosum) is a widely distributed, nutrient-rich food and vegetable crop, and it is the fourth largest crop after wheat, rice and corn in my country. [0003] At present, plant tissue culture technology is mainly used to breed improved potato varieties and virus-free potatoes. This technology needs to achieve the purpose of rapid multiplication of potato microtubers through subculture. The reproduction coefficient of subculture is high, and a large number of seedlings can be obtained quickly, but there are some disadvantages in the expansion of seedlings through tissue culture: first, the equipment requirements are high, and a professional tissue culture laboratory is required; The technical operation level is relat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G22/25
CPCA01G22/00
Inventor 毛碧增曾欣
Owner ZHEJIANG UNIV
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