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Verticillium dahliae pathogenicity test and evaluation method

A technology for cotton Verticillium wilt bacteria and an evaluation method, which is applied in the field of virulence determination and evaluation of cotton Verticillium wilt bacteria, can solve the problems such as no unified pathogenicity determination and evaluation method, no comparability, etc., achieves easy mastery and avoids time-consuming The effect of laborious, experimental results science

Inactive Publication Date: 2017-04-26
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the pathogenicity of Verticillium dahliae of cotton is differentiated in various ways, and there is no unified pathogenicity measurement and evaluation method. Various researchers use different identification hosts, disease investigation methods, and pathogenicity evaluation methods. comparability

Method used

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  • Verticillium dahliae pathogenicity test and evaluation method
  • Verticillium dahliae pathogenicity test and evaluation method
  • Verticillium dahliae pathogenicity test and evaluation method

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Experimental program
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Effect test

Embodiment 1

[0023] Embodiment 1 Verticillium dahliae pathogenicity assay and evaluation method

[0024] 1. Preparation of strains to be tested

[0025] 1.1 Isolation and morphological identification of strains to be tested

[0026] Before mid-August, collect the middle and lower parts of the main stems of cotton verticillium wilt diseased plants, cut them into small sections of about 4 cm, remove the epidermis, and disinfect with 75% alcohol. Soak the peeled stems in 0.1% mercury liter for about 30-60 minutes on the ultra-clean workbench, adjust the disinfection time according to the thickness of the stems, wash 3 times with sterilized distilled water, and cut into 2-3mm Flakes. Place the slice in the center of a PDA petri dish and culture it in the dark at 25°C. On the third day of cultivation, off-white hyphae grow at both ends of the cut cotton stem tissue, forming off-white colonies called wild colonies. Pick a small amount of mycelia Observed under a microscope, there are typical ...

Embodiment 2

[0069] Embodiment 2 Cotton verticillium dahliae pathogenicity measurement and verification of evaluation method of the present invention

[0070] 1. Bacterial strains for testing: select bacterial strains T-9, SS-4, VD-8 and V991, Vd076, Vd080 and Vd171 (Table 4) that are used more in the prior art.

[0071] Table 4 Basic information of commonly used strains

[0072]

[0073] Note: V991 is derived from articles by Wang Limei et al. (Journal of Phytopathology, 1999, 29(2): 181~189), T9, SS-4 and VD-8 are derived from articles by Lu Jiayun et al. (Journal of Nanjing Agricultural University, 1983, (1) : 36~43)

[0074] 2. Pathogenicity evaluation

[0075] The selected two representative existing pathogenicity evaluation methods were compared with the method established by the present invention.

[0076] Method 1: From Jin Lirong et al. (Journal of Hubei University, 2016, 38(2): 120-126, 134). With Lumianyan 28, Yumian 21 and Jimian 11 as differential hosts, the method of i...

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Abstract

The invention relates to the field of cotton disease control, in particular to a verticillium dahliae pathogenicity test and evaluation method. The method comprises the steps of inoculating to-be-tested verticillium dahliae and a control strain with differential hosts: performing disease investigation; and obtaining a corrected disease index based on an investigation result when a disease index of the control strain in correcting the differential hosts reaches 45.0-55.0. The method is simple and easy to master, is short in identification cycle, and is scientific, accurate and standard.

Description

technical field [0001] The invention relates to the field of cotton disease prevention and control, in particular to a method for measuring and evaluating the pathogenicity of Verticillium dahliae of cotton. Background technique [0002] Cotton verticillium wilt is one of the main diseases of cotton. Cotton verticillium wilt spreads rapidly and causes serious economic losses. It has become one of the main limiting factors for the development of cotton production in my country. Theoretical research and production practice have proved that the breeding and utilization of disease-resistant varieties is the most economical and effective means to control the disease. However, during the process of co-evolution of Verticillium dahliae and the host, new pathogenic types often appear, making the disease-resistant varieties The loss of resistance leads to widespread prevalence of Verticillium wilt. Therefore, continuous research on its pathogenicity differentiation is of great signif...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12Q1/04C12R1/645
CPCC12Q1/04C12Q1/18
Inventor 袁媛朱荷琴冯自力冯鸿杰李志芳师勇强赵丽红魏锋
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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