Method of improving mogroside IIE content

A technology of mogroside and Luo Han Guo, applied in the field of plant biology, can solve the problems that restrict the healthy development of the Luo Han Guo industry, and have not seen any improvement in the content of Mogroside ⅡE, low content, etc. Effect

Inactive Publication Date: 2017-05-10
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have found that glucoside V is transformed from glucoside IIE and other low glycosides, especially in the later stage of fruit development, glucoside IIE and other low glycosides are completely consumed and converted into glucoside V, glucoside IIE is also an important active ingredient in Luo Han Guo, However, glycoside IIE only exists in the pulp which accounts for less than 15% of the fruit weight, and the content is extremely low, which seriously restricts the healthy development of the Luo Han Guo industry.
There is no report on increasing the content of mogroside IIE in the prior art

Method used

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  • Method of improving mogroside IIE content

Examples

Experimental program
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Effect test

Embodiment 1

[0034] A method for increasing the content of mogroside IIE is to inoculate the mogroside tissue culture seedlings in an induction treatment medium for induction treatment and culture for 28 days, wherein the concentration of methyl jasmonate in the induction treatment medium is 50 μmol / L.

[0035] Wherein, the induction treatment medium also includes a basic medium, and its formula is: MS+1.5mg / L 6-BA+0.3mg / L IBA+3.5g / L agar+30g / l sucrose+1.0g / L Activated carbon.

[0036] Wherein, the conditions of the induction treatment culture are: relative humidity 60-66%, light intensity 1400 lux, light time 8h / d, temperature 23±2°C.

[0037] Among them, the preparation method of the induction treatment medium is as follows: dissolve methyl jasmonate in a 2% ethanol aqueous solution, prepare a 50 mmol / L methyl jasmonate mother liquor, and sterilize it with a 0.22 μm microporous membrane. The sterilized methyl jasmonate mother liquor is added to the basic medium until the methyl jasmonate conce...

Embodiment 2

[0039] A method for increasing the content of mogroside IIE is to inoculate the mogroside tissue culture seedlings in an induction treatment medium for induction treatment and culture for 35 days, wherein the concentration of methyl jasmonate in the induction treatment medium is 400 μmol / L.

[0040] Wherein, the induction treatment medium also includes a basal medium, the formula of which is: MS+1.5mg / L 6-BA+0.3mg / L IBA+3.5g / L agar+30g / l sucrose+1.0g / L Activated carbon.

[0041] Wherein, the conditions of the induction treatment culture are: relative humidity 60-66%, light intensity 1400 lux, light time 8h / d, temperature 23±2°C.

[0042] Among them, the preparation method of the induction treatment medium is as follows: dissolve methyl jasmonate in a 2% ethanol aqueous solution, prepare a 50 mmol / L methyl jasmonate mother liquor, and sterilize it with a 0.22 μm microporous membrane. The sterilized methyl jasmonate mother liquor is added to the basic medium until the methyl jasmonate...

Embodiment 3

[0044] A method for increasing the content of mogroside IIE is to inoculate the mogroside tissue culture seedlings in an induction treatment medium for induction treatment and culture for 30 days, wherein the concentration of methyl jasmonate in the induction treatment medium is 300 μmol / L.

[0045] Wherein, the induction treatment medium also includes a basic medium, and its formula is: MS+1.5mg / L 6-BA+0.3mg / L IBA+3.5g / L agar+30g / l sucrose+1.0g / L Activated carbon.

[0046] Wherein, the conditions of the induction treatment culture are: relative humidity 60-66%, light intensity 1400 lux, light time 8h / d, temperature 23±2°C.

[0047] Among them, the preparation method of the induction treatment medium is as follows: dissolve methyl jasmonate in a 2% ethanol aqueous solution, prepare a 50 mmol / L methyl jasmonate mother liquor, and sterilize it with a 0.22 μm microporous membrane. The sterilized methyl jasmonate mother liquor is added to the basic medium until the methyl jasmonate conc...

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Abstract

The present invention discloses a method of improving mogroside IIE content. Siraitia grosvenorii seedlings after tissue culture are inoculated into an induction treatment culture medium to be subjected to the induction treatment culture for 28-35 days. The concentration of jasmonic acid methyl ester in the induction treatment culture medium is 50-400 [mu]mol/L. The jasmonic acid methyl ester is used to treat the siraitia grosvenorii seedlings after the tissue culture to induce a high expression of key enzyme genes of mogroside IIE metabolism, thereby improving the content of the mogroside IIE.

Description

Technical field [0001] The invention relates to the field of plant biotechnology. More specifically, the present invention relates to a method for increasing the content of mogroside IIE. Background technique [0002] Luo Han Guo (Siraitia grosvenorii) is a precious medicinal and sweetener plant unique to China. The fruit is cool in nature, sweet in taste, has the effects of clearing heat and moisturizing the lungs, stimulating the sound of the pharynx, smoothing the bowel, and anti-cancer. The active ingredient glycoside V is one of the strongest non-sugar sweeteners in the world. It is sweetened with sucrose. It is 300-400 times higher than the degree, widely used in food, health care products and medicines, and is an ideal sugar substitute for diabetic, obese and hypertensive patients. Studies have found that Glycoside V is transformed from glycosides such as Glycoside IIE. Especially in the later stages of fruit development, Glycoside IIE and other low-glycosides are comple...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G9/10A01G9/08A01G7/06
CPCA01G7/06A01G9/029A01G9/0299A01G9/086A01G24/00A01H4/001A01H4/008
Inventor 韦荣昌
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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