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Separation, purification and primary structure identification method of dendrobium devonianum homogeneous polysaccharides

A technology of dendrobium dendrobium polysaccharides, applied in the field of dendrobium polysaccharides, can solve the problems of high molecular weight, high viscosity, separation and purification structure analysis of highly active polysaccharides, etc.

Active Publication Date: 2017-05-10
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high viscosity of Dendrobium dendrobii polysaccharides and the large molecular weight of highly active polysaccharides, there are certain difficulties in separation, purification and structural analysis.

Method used

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  • Separation, purification and primary structure identification method of dendrobium devonianum homogeneous polysaccharides
  • Separation, purification and primary structure identification method of dendrobium devonianum homogeneous polysaccharides
  • Separation, purification and primary structure identification method of dendrobium devonianum homogeneous polysaccharides

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Experimental program
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Effect test

Embodiment 1

[0027] Embodiment 1: Separating and purifying the homogeneous polysaccharide of Dendrobium dendrobium by the following steps: decoct Dendrobium dendrobium in water, and carry out solid-liquid separation to obtain a water extract; add ethanol alcohol precipitation to the water extract to make the ethanol concentration in the system be 70%, stand overnight to obtain crude polysaccharide; redissolve the crude polysaccharide in water to completely dissolve the polysaccharide in water, add alcohol to 50% for ethanol precipitation, obtain a precipitate, remove the precipitate, continue to add alcohol to The system alcohol is 70%, and the 50-70% alcohol-precipitated crude polysaccharide fragments with a purity of more than 90% are obtained; the crude polysaccharide fragments are redissolved in water, purified with superdex-200, tracked and collected by the sulfuric acid phenol method, and the symmetrical peak 33- 45 tubes were concentrated by a rotary evaporator for 50 minutes, dialyz...

Embodiment 2

[0028] Embodiment 2: Separating and purifying the homogeneous polysaccharide of Dendrobium dendrobium by the following steps: decocting Dendrobium dendrobium in water, and carrying out solid-liquid separation to obtain a water extract; adding ethanol alcohol precipitation to the water extract to make the ethanol concentration in the system be 80%, stand overnight to obtain crude polysaccharide; redissolve the crude polysaccharide in water to completely dissolve the polysaccharide in water, add alcohol to 50% for ethanol precipitation, obtain a precipitate, remove the precipitate, continue to add alcohol to The system alcohol is 70%, and the 50-70% alcohol-precipitated crude polysaccharide fragments with a purity of more than 90% are obtained; the crude polysaccharide fragments are redissolved in water, purified with superdex-200, tracked and collected by the sulfuric acid phenol method, and the symmetrical peak 35- 42 tubes were concentrated with a rotary evaporator for 50 minu...

Embodiment 3

[0029] Embodiment 3: The obtained homogeneous polysaccharide is subjected to structural analysis through the following steps:

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Abstract

The invention discloses a method for separating and purifying dendrobium devonianum homogeneous polysaccharides and aims to solve the problem that the separation, purification and structural analysis of dendrobium devonianum polysaccharides have certain difficulty due to the fact that the dendrobium devonianum polysaccharides are high in viscosity and large in high-activity polysaccharide segment molecular weight. The method includes: separating with a step-by-step ethanol precipitation method, and purifying with a gel column to obtain pure the dendrobium devonianum homogeneous polysaccharides. In addition, the invention further uses multispectral analysis to identify the skeletal structure of the dendrobium devonianum homogeneous polysaccharides, the primary structure of the dendrobium devonianum homogeneous polysaccharides can be identified accurately, and the main polysaccharides in dendrobium devonianum can be determined as acetyl glucomannan.

Description

technical field [0001] This case involves the field of dendrobium polysaccharides, especially a method for separating and purifying dendrobium dendrobium polysaccharides, and a method for identifying the primary structure of the polysaccharides. Background technique [0002] Polysaccharides are important biologically active macromolecules, which have the functions of improving immunity, anti-inflammation, delaying aging, and anti-oxidation. A large number of hydrophilic hydroxyl groups linked by polysaccharides make it highly water-absorbing, emulsifying, and high-viscosity. [0003] Dendrobium dendrobii is the first of the nine immortal grasses. Dendrobium dendrobii polysaccharides have good effects of improving immunity and anti-tumor. Its polysaccharide content is rich, as high as 35-42%. Previous experiments have found that Dendrobium dendrobium polysaccharides have more significant anti-tumor and immunity-enhancing effects than Ganoderma lucidum polysaccharides. At pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00G01N30/02G01N30/88G01N24/00
CPCC08B37/0003C08B37/0087G01N24/00G01N30/02G01N30/88G01N2030/027
Inventor 王东晖王凤忠方芳来吉祥王艳黄璐璐
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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