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Blocking agent kit for immunochromatography determination and using method thereof

A technology of immune chromatography and blocking agents, which is applied to measuring devices, analytical materials, instruments, etc., can solve the problems of insufficient blocking effect of blocking agents and the inability to completely prevent the interference of endogenous factors, and achieve a good blocking effect , the effect of improving accuracy

Active Publication Date: 2017-05-10
GUANGZHOU KEFEN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, studies have found that in some cases, the blocking effect of these blocking agents added to commercial immune reagents is not sufficient to completely prevent the interference of endogenous factors on the test results

Method used

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  • Blocking agent kit for immunochromatography determination and using method thereof
  • Blocking agent kit for immunochromatography determination and using method thereof
  • Blocking agent kit for immunochromatography determination and using method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-4

[0034] A blocking agent kit for immunochromatographic determination, comprising a box body and a blocking agent arranged in the box body, the blocking agent includes a biological blocking agent and a chemical blocking agent, and the biological blocking agent It is set in the box body separately from the chemical blocking agent. Wherein the contents of each component in the biological blocking agent and the chemical blocking agent are shown in Table 1.

[0035] The concentration table of each component of compound blocking agent in the embodiment 1-4 of table 1

[0036]

[0037]

Embodiment 5-7

[0039] A blocking agent kit for immunochromatographic determination, a blocking agent kit for immunochromatographic determination, comprising a box body and a blocking agent arranged in the box body, the blocking agent includes biological The blocking agent and the chemical blocking agent, the biological blocking agent and the chemical blocking agent are separately arranged in the box body. The contents of each component in the biological blocking agent and the chemical blocking agent are shown in Table 2.

[0040] Concentration table of each component of composite blocker in table 2 embodiment 5-7

[0041]

[0042]

experiment example 1

[0043] Experimental Example 1: Quantitative determination of cardiac troponin I (cTnI) by fluorescence immunochromatography

[0044]8 cases of human serum samples (experimental samples, specimen number 1-8) confirmed to have obvious endogenous interference and 2 cases of human serum samples confirmed to have no obvious endogenous interference (control samples, specimen number 9-10) were selected as the research Object, prepare sample pad treatment solution and sample diluent according to the method recorded in "Establishment of Colloidal Gold Immunochromatography for Rapid Detection of Avian Influenza Virus and Serum Antibody" (Feng Juan, Yangzhou University, 2006), and then take Example 1 -4 Composite blocking agent prepared by adding the biological blocking agent to the sample pad treatment solution at a ratio of 0.5vol%, and adding the chemical blocking agent to the sample diluent at a ratio of 0.5vol%, and then according to YY / T1221 -The method stipulated in 2013 (cardiac ...

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Abstract

The invention discloses a blocking agent kit for immunochromatography determination. The blocking agent kit comprises a kit body and a blocking agent arranged in the kit body. The blocking agent comprises a biological blocking agent and a chemical blocking agent. The biological blocking agent and the chemical blocking agent are separated to be arranged in the kit body. The invention further discloses a method for using the blocking agent kit. According to the blocking agent kit for immunochromatography determination, blocking effects of the biological blocking agent and the chemical blocking agent under endogenous interference factors of different concentrations are brought into full play, the influence on immunochromatography determination caused by the endogenous interference factors in a sample can be effectively prevented and controlled, accuracy of immunochromatography quantitative determination results is improved, and the blocking agent kit has better suitability.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a blocking agent kit for quantitative detection of immunochromatography and a use method thereof. Background technique [0002] With the progress of society and the development of medicine, major changes have taken place in the functions and tasks of hospitals, from the simple treatment type in the past to the comprehensive type integrating multiple functions such as prevention, health care, treatment and rehabilitation, providing comprehensive services for the society. quality service. Hospital work has also expanded from a single in-hospital medical care to out-of-hospital communities and even remote mountainous areas. In order to better realize the functions of the hospital, an accurate, sensitive, and convenient point-of-care test (POCT) is needed. [0003] Immunochromatography (Immunochromatography) is a new type of immunodiagnostic technology developed in the early 1980s. Th...

Claims

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Application Information

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IPC IPC(8): G01N33/531
CPCG01N33/531
Inventor 孙子洪何明深
Owner GUANGZHOU KEFEN BIOTECH CO LTD
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