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Immune cell cryopreservation liquid and application thereof

A technology of immune cells and cryopreservation solution, which is applied in the field of cell culture, can solve the problems of discarding and ineffective preservation of immune cells, and achieve the effect of large cell volume and good cell cryopreservation effect

Active Publication Date: 2017-05-17
广州沙艾生物科技有限公司
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  • Abstract
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  • Claims
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Problems solved by technology

[0004] At present, the existing cell cryopreservation solution cannot effectively preserve large-scale cultured immune cells, and the number of cells and cell viability after recovery of the cryopreserved cells cannot meet the standards for clinical application, resulting in the large-scale culture of immune cells in vitro. Apply as soon as possible within the time limit, otherwise it can only be discarded
[0005] Therefore, the in vitro cryopreservation solution suitable for a large number of immune cells needs to be improved

Method used

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  • Immune cell cryopreservation liquid and application thereof

Examples

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Embodiment 1

[0044] In this example, 6 kinds of cryopreservation solutions without plasma and 6 kinds of cryopreservation solutions containing plasma were used to freeze the immune cells induced and amplified in vitro, and the effects of freezing were compared, so as to observe the effect of plasma on immune cell freezing. effects on preservation, and the possibility of lowering the DMSO concentration to reduce cytotoxicity. details as follows:

[0045] 1. Use plasma-free cryopreservation medium to freeze immune cells

[0046] 1.1. Isolate and obtain umbilical cord blood mononuclear cells, inoculate the mononuclear cells into a culture bottle with 20ml of medium, and the ratio of the medium is: 18ml OpTmizer TM CTS TM T-cell expansion SFM medium + 2ml autologous plasma (Autologous plasma) + final concentration 1000IU / ml Pepro Tech IL-2 + final concentration 0.01KE / ml Sapelin (OK432), 37°C, 5% CO 2 Aseptic culture was recorded as day 0. Observe the cells every day, and carry out appropr...

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Abstract

The invention discloses immune cell cryopreservation liquid and application thereof. The immune cell cryopreservation liquid is prepared from 2 volume percent to 10 volume percent of DMSO (Dimethylsulfoxide), 2 volume percent to 15 volume percent of HSA (Human Serum Albumin), 20 volume percent to 88 volume percent of blood serum, conyza japonica saponin R which is selectively added and the balance of a culture medium. The cryopreservation liquid disclosed by the invention can be used for effectively preserving immune cells and the preservation time is long; after the cells are recovered, the recovery capability is high and the recycling rate of the cells is high.

Description

technical field [0001] The invention relates to an immune cell cryopreservation solution and application thereof, belonging to the field of cell culture. More specifically, it relates to an immune cell cryopreservation solution, a kit for freezing immune cells and a method for freezing immune cells. Background technique [0002] As an effective method to preserve cells, cell freezing technology has been widely used in the field of biology. Cryopreservation of donor immune cells can maintain their vitality and function, which is of great significance for both clinical and basic research, especially when immune cells are used for retrospective research. Cryopreservation of immune cells can not only solve the problem of long induction time of existing immune cells, multiple inductions and multiple blood collections from patients, but also preserve immune cells in the best health state or the strongest combat effectiveness period for use in tumors treatment and anti-aging heal...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0284
Inventor 徐智峰王辉
Owner 广州沙艾生物科技有限公司
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