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Application of Rice Gene osuep3 and Its Regulatory Function of Disease Resistance

A gene, rice technology, applied in the field of disease resistance regulation function analysis of rice genes, to achieve the effects of high disease resistance varieties, a wide range of available disease resistance resources, and strong resistance

Active Publication Date: 2020-01-03
ZHEJIANG UNIV
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These point mutations resulted in no amino acid changes

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  • Application of Rice Gene osuep3 and Its Regulatory Function of Disease Resistance

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Embodiment 1

[0028] In the present invention, a rice gene OsUEP3 is cloned, and for the first time by constructing over-expression transgenic rice, the regulation function of the gene in broad-spectrum resistance to different strains of bacterial blight is clarified. Simultaneously, the overexpression transgenic rice constructed by the present invention has enhanced disease resistance, therefore, the present invention provides a set of overexpression transgenic rice by constructing the gene, and adopting genetic engineering technology to create and obtain broad-spectrum resistance to rice bacterial diseases. material technology system. The main steps include:

[0029] 1) Cloning and preservation of rice OsUEP3 gene

[0030]The rice OsUEP3 gene provided by the invention is obtained by cloning through the following steps. First, the primer OsUEP3-F3 (5'-caggcgcgcc atg cag atc ttc gtc aag acc-3', the part in italics is the Asc I restriction site) was designed according to the OsUEP3 sequenc...

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Abstract

The invention discloses oryza sativa gene OsUEP3. The open reading frame of the gene is 390 bp in length, and the gene encodes a ubiquitin extension protein and comprises 129 amino acids. Ubiquitin molecules of 76 amino acids are arranged at the N terminal of the protein, and 60S ribosomal protein L40e of 53 amino acids are arranged at the C terminal of the protein. The regulation effect of the gene on the bacterial disease resistance of oryza sativa is illuminated for the first time by constructing an overexpression transgenic oryza sativa of the gene and analyzing disease resistance. The resistance of the overexpression transgenic oryza sativa plants to Xanthomonas oryzae strains PXO99 and PXO112 is improved remarkably, so that the positive regulation function of the gene for the Xanthomonas oryzae resistance is revealed, and the regulation function has broad spectrum for different strains. The oryza sativa gene OsUEP3 is a novel gene resource suitable for creating and breeding novel oryza sativa materials and novel varieties with broad-spectrum resistance to Xanthomonas oryzae and other bacterial diseases.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the analysis of the disease resistance regulating function of a rice gene (OsUEP3) and its application. Background technique [0002] 1. Plant gene cloning technology [0003] There are many methods for plant gene cloning. With the increase of plant species whose genome sequence has been determined, the method of plant gene cloning based on database sequence has been more and more widely used. The operation steps of the method mainly include primer design based on conserved sequences, RNA extraction from target plant tissue, reverse transcriptase-polymerase chain reaction (RT-PCR), connection of PCR product and vector, bacterial transformation of the connection product, and plasmid extraction. , enzyme digestion test, sequencing analysis, etc. [0004] 2. Plant gene expression control and gene function analysis technology [0005] The function of plant genes is carried out through g...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/82C07K14/415A01H5/00A01H6/46
CPCC07K14/415C12N15/8205C12N15/8216C12N15/8281
Inventor 蔡新忠徐幼平郭伟毅
Owner ZHEJIANG UNIV
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