Artificial antibody for targeting EGFR (epidermal growth factor receptor) based on gold nanoparticles and preparation method thereof

A technology of epidermal growth factor and gold nanoparticles, applied in the direction of anti-receptor/cell surface antigen/cell surface determinant immunoglobulin, chemical instruments and methods, anti-animal/human immunoglobulin, etc., can solve the preparation cost High, low binding force, poor stability and other problems, to achieve broad application prospects, strong binding force, good stability

Active Publication Date: 2017-05-24
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the nanomedicine synthesized by this method has good tumor targeting, the nanomedicine synthesized by this method has low binding force to EGFR, poor stability and high preparation cost due to the difficulty in extracting macromolecular protein antibodies and poor stability.

Method used

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  • Artificial antibody for targeting EGFR (epidermal growth factor receptor) based on gold nanoparticles and preparation method thereof
  • Artificial antibody for targeting EGFR (epidermal growth factor receptor) based on gold nanoparticles and preparation method thereof
  • Artificial antibody for targeting EGFR (epidermal growth factor receptor) based on gold nanoparticles and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] 1. Design of peptide fragments

[0042] Directly combine the recognition region and the fixed region, the number of amino acids in the regulatory region is 0, and the polypeptide P1:CAWYGTLYEYDC is designed, such as figure 1 shown. The designed polypeptide P1 can be fixed on the surface of gold nanoparticles through the sulfhydryl groups in the cysteines at both ends, thereby forming a specific conformation.

[0043] 2. Preparation of gold nano-artificial antibody AuNP(4nm)+P1 with the ability to target EGFR

[0044] According to 1nm 2The ratio of 0.05-2 polypeptides is fixed on the surface area of ​​the nano-gold sphere, and the polypeptide is fixed on the surface of the 4nm gold nano-sphere through the S-Au bond, and each nano-gold surface has 10-150 polypeptides. Thus, the artificial antibody AuNP(4nm)+P1 capable of specifically binding to EGFR was obtained.

[0045] 3. To characterize the specific binding performance of the artificial antibody AuNP (4nm) + P1 to...

Embodiment 2

[0052] 1. Design of peptide fragments

[0053] A serine and a glycine were added to the two regulatory regions at both ends of the recognition region, and the polypeptide P2:CSAWYGTLYEYDGC was designed, such as figure 1 shown. The designed polypeptide can be immobilized on the surface of gold nanoparticles through the sulfhydryl groups in the cysteine ​​of the two immobilization regions, thereby forming a specific conformation.

[0054] 2. Preparation of gold nanoartificial antibody with the ability to target EGFR

[0055] According to 1nm 2 The ratio of 0.05-2 polypeptides is fixed on the surface area of ​​gold nanoparticles, and the polypeptides are fixed on the surface of 4nm nano-gold spheres through S-Au bonds, and each nano-gold surface has 10-150 polypeptides. Thus, the artificial antibody AuNP(4nm)+P2 capable of specifically binding to EGFR was obtained.

[0056] 3. To characterize the specific binding performance of the artificial antibody AuNP (4nm) + P2 to the p...

Embodiment 3

[0063] 1. Design of peptide fragments

[0064] Add 5 amino acids to each of the two regulatory regions at both ends of the recognition region, and design the polypeptide P3: CGAGASAWYGTLYEYDGSASAC, such as figure 1 shown. The designed polypeptide can be immobilized on the surface of gold nanoparticles through the sulfhydryl groups in the cysteine ​​of the two immobilization regions, thereby forming a specific conformation.

[0065] 2. Preparation of gold nanoartificial antibody with the ability to target EGFR

[0066] According to 1nm 2 The ratio of 0.05-2 polypeptides is fixed on the surface area of ​​gold nanoparticles, and the polypeptides are fixed on the surface of 4nm nano-gold spheres through S-Au bonds, and each nano-gold surface has 10-150 polypeptides. Thus, the artificial antibody AuNP(4nm)+P3 capable of specifically binding to EGFR was obtained.

[0067] 3. To characterize the specific binding performance of the artificial antibody AuNP (4nm) + P3 to the protei...

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Abstract

The invention relates to an artificial antibody for targeting an EGFR (epidermal growth factor receptor) based on gold nanoparticles and a preparation method thereof. The artificial antibody is characterized by consisting of the gold nanoparticles and a section of designed polypeptide sequence; the conformation of the polypeptide on the nanometer gold surface is reconstructed, so as to prepare the artificial antibody based on the gold nanoparticles. The artificial antibody has the advantages that the property of specifically identifying the EGFR is realized, the bonding ability is strong, and the stability is good; the artificial antibody is used for specially targeting the over-expressed EGFR on the tumor cell surface, and the artificial antibody with the special EGFR identifying function has broad application prospect in the fields of disease diagnosis, targeting therapy, photo-thermal therapy, and medicine carriers.

Description

technical field [0001] The invention relates to an artificial antibody targeting epidermal growth factor receptor EGFR (epidermal growth factor receptor) and a preparation method thereof. The feature is an artificial antibody targeting epidermal growth factor receptor EGFR based on gold nanoparticles and a preparation method thereof. Background technique [0002] Targeted delivery of nanoparticles to tumor sites is crucial for nanomedicine in the fields of in vivo molecular imaging and targeted therapy. In recent years, in order to solve the problem of poor binding force and low specificity between nanoparticles and target sites, more and more people use the high specificity and strong binding force of antibodies and antigens by binding natural antibodies to nanoparticles. properties, so that the nanoparticles are directed to the target location and can prolong the circulation time of the nanoparticles in the body. [0003] Studies have found that EGFR is highly expressed ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/28
CPCC07K16/2863C07K2317/569
Inventor 曹傲能汪坤王海芳
Owner SHANGHAI UNIV
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