Method for regulating fermenting microorganisms using epigenetic modification

A technology for epigenetic modification and fermentation of microorganisms, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low activity and low amount of substances, and achieve low biological activity, increased activity, and low cost. cheap effect

Active Publication Date: 2020-09-15
ZHEJIANG OCEAN UNIV
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Problems solved by technology

However, in the case of natural fermentation, the symbiotic microorganisms of Enteromorpha secrete less algae and antibacterial substances, and the activity is not large.

Method used

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  • Method for regulating fermenting microorganisms using epigenetic modification

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Embodiment 1

[0017] The method of using epigenetic modification to regulate fermentation microorganisms, the specific operation steps are:

[0018] 1) Expansion cultivation: Inoculate Aspergillus terreus in the sterilized and cooled medium for expansion cultivation. The composition of the expansion medium and its parts by weight are: 15~25 parts of mannitol, 2~5 parts of yeast extract, 0.1~0.8 parts of sulfuric acid Magnesium, 8~15 parts of monosodium glutamate, 9~20 parts of glucose, 02~1.1 parts of potassium dihydrogen phosphate, 18~25 parts of maltose, 0.8~1.5 parts of corn steep liquor, 30~40 parts of seawater. The culture temperature is 25-40°C, the pH is 6.5-7.5, and the culture time is 20-40 days. The above medium can well satisfy the nutrients and growth factors required for the growth and reproduction of the symbiotic microorganisms of Enteromorpha enteromorpha, accelerate its growth and reproduction, and shorten the time required for the experiment;

[0019] 2) Epigenetic modifi...

Embodiment 2

[0021] The method for using epigenetic modification to regulate fermenting microorganisms, the most preferred steps are:

[0022] 1) Expansion cultivation: Inoculate Aspergillus terreus in the sterilized and cooled medium for expansion cultivation. The composition of the expansion medium and its most preferred parts by weight are: 20 parts of mannitol, 3 parts of yeast extract, 0.3 parts of magnesium sulfate, 10 parts of MSG, 12 parts of glucose, 0.5 parts of potassium dihydrogen phosphate, 20 parts of maltose, 1.0 part of corn steep liquor, 33 parts of seawater. The culture temperature was 28°C, the pH was 7.0, and the culture time was 30 days. The above-mentioned medium can well meet the nutrients and growth factors required for the growth and reproduction of the symbiotic microorganisms of Enteromorpha enteromorpha, accelerate its growth and reproduction, and shorten the time required for the experiment;

[0023] 2) Regulation of epigenetic modification: add fungal liquid cu...

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Abstract

The invention provides a method for using epigenetic modification to regulate fermentation microorganisms, that is, adding histone deacetylase inhibitor TSA (trichostatin A) to perform epigenetic modification to regulate fermentation. The beneficial effect is: the fermenting microorganism regulated by the epigenetic modification, due to the activation of the expression of the silenced gene, the activity of the metabolites inhibiting Vibrio anguillarum is significantly improved, and the secondary metabolites are obviously abundant.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for using epigenetic modification to regulate and control fermenting microorganisms. Background technique [0002] Zhoushan's marine aquaculture industry mainly focuses on characteristic and advantageous species such as large yellow croaker, swimming crab, prawn, mussel and black sea bream, and mainly focuses on shallow sea aquaculture and pond aquaculture. With the expansion of aquaculture scale, the intensity of aquaculture is increasing, and the ecological environment of aquaculture is also deteriorating. At the same time, the diseases of aquaculture industry are becoming more and more serious, which has caused great economic losses to fishermen. Among them, the main disease of large-scale aquaculture is caused by Vibrio anguillarum ( Vibrio anguillarum ) infection caused by vibriosis, the disease spreads rapidly, the infection rate is high, and the mortality rate is as ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/02C12R1/66
CPCC12P1/02C12N1/145C12R2001/66
Inventor 孙坤来刘文韩鹏杰韩烨华陈荫赵玉勤王兵
Owner ZHEJIANG OCEAN UNIV
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