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Application of SUSD2 in preparation of kit for diagnosis and/or prognostic judgement of high-grade serous ovarian carcinoma

A prognostic judgment, ovarian cancer technology, applied in the field of biomedicine, can solve the problems of low long-term survival rate and poor prognosis

Inactive Publication Date: 2017-05-24
SHANDONG UNIV QILU HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

HGSOC has a poor prognosis, with long-term survival rates below 20%

Method used

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  • Application of SUSD2 in preparation of kit for diagnosis and/or prognostic judgement of high-grade serous ovarian carcinoma
  • Application of SUSD2 in preparation of kit for diagnosis and/or prognostic judgement of high-grade serous ovarian carcinoma
  • Application of SUSD2 in preparation of kit for diagnosis and/or prognostic judgement of high-grade serous ovarian carcinoma

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Experimental program
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Effect test

Embodiment 1

[0033] 1. Test method:

[0034] We collected archival wax blocks of surgical specimens from 55 patients with fimbria and 306 patients with high-grade serous ovarian cancer from 2007-2013. Using a semi-automatic tissue chip punching machine to make 3 TMA tissue chips to ensure large samples, high throughput and standardization of immunohistochemistry. Each block contains fimbria and high-grade serous ovarian carcinoma.

[0035] Tissue Microarray (TMA) technology is a microarray tissue sheet made by neatly arranging dozens to hundreds or even more tissue samples on a glass slide. Through immunohistochemistry, in situ hybridization, etc., high-throughput acquisition of genomics and proteomics expression information based on morphology has been achieved on tissue sections. Tissue chip technology has been widely used in tumor research to study the changes of genes and proteins in different development stages of tumors, the evaluation of prognosis, the evaluation of molecular mark...

Embodiment 2

[0074] The method of the embodiment of the present invention 1 is adopted to detect the tissue of the wax block specimen, and the microscopic examination results are as follows: image 3 and Figure 4 shown. in, image 3 The expression of SUSD2 in the tissue section shown is relatively high, and there are obvious brown particles on the cell membrane, which is a positive result of SUSD2 detection, and the result of pathological examination is high-grade serous ovarian cancer.

[0075] Figure 4 The expression level of SUSD2 in the shown tissue section is low, without obvious brownish-yellow granules, which is a negative result of SUSD2 test, and the result of pathological test is negative for lymph node metastasis.

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Abstract

The invention discloses the application of SUSD2 as a marker for the diagnosis and / or prognostic judgement of high-grade serous ovarian carcinoma and application for detection of an SUSD2 reagent in preparation of a kit for the diagnosis and / or prognostic judgement of the high-grade serous carcinoma. In the invention, SUSD2 is used as the marker to diagnose high-grade serous ovarian carcinoma through a method of immunohistochemistry and judges the prognosis of the high-grade serous ovarian carcinoma; the SUSD2 is highly expressed in the high-grade serous ovarian carcinoma and lowly expressed in fimbriae of fallopian tube. The expression level of SUSD2 in high-grade ovarian carcinoma is related to the survival rate of patients; patients having high SUSD2 expression are poor in prognosis and low in survival rate. The SUSD2 as the marker for the diagnosis and / or prognosis of the high-grade serous ovarian carcinoma has broad application prospects and huge potential social benefits.

Description

technical field [0001] The invention relates to the field of biomedical technology, in particular to the application of SUSD2 in preparing a kit for diagnosis and / or prognosis judgment of high-grade serous ovarian cancer. Background technique [0002] Ovarian cancer is a common malignant tumor in gynecology, and its case fatality rate ranks first among gynecological malignant tumors. It is the biggest challenge facing obstetrics and gynecology. Although tumor cytoreductive surgery and platinum-taxane combination chemotherapy have improved the 5-year survival rate of patients, there has been no substantial progress in the clinical diagnosis and treatment of ovarian cancer for half a century. This is mainly due to the lack of clinically practical early diagnosis methods and long-lasting treatment options. The fundamental reason is that the molecular mechanisms of early diagnosis, development, and prognosis of ovarian cancer are not clearly understood. [0003] High-grade sero...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/68
CPCG01N33/57449G01N33/6893
Inventor 孔北华徐莹张青李宜诺程傲霜孙晓梅
Owner SHANDONG UNIV QILU HOSPITAL