Nanometer-silver lipidosome and preparing method and application thereof

Abstract

The invention provides a nanometer-silver lipidosome and a preparing method and application thereof, and relates to the technical field of lipidosomes. The nanometer-silver lipidosome is mainly prepared from nanometer silver, lecithin, cholesterol, an antioxygen, a metal complexing agent, an emulsifying agent, an anti-polymerizing agent, water and the like; the nanometer silver is wrapped with the lipidosome, the capacity breaking through human-cell barriers of the nanometer silver is improved through the synergistic effect of the nanometer silver and the lipidosome, and therefore the speed of the nanometer silver entering microbial cells is greatly increased; the high-concentration drug effect is formed on the focus periphery in a gathered mode, and the rapid and lasting bactericidal aim is achieved while the safety is guaranteed. The invention also provides the preparing method for the nanometer-silver lipidosome; the preparing method includes the steps that all the raw materials are mixed, homogenized and then subjected to ultrahigh-pressure micro-jet circular treatment with an ultrahigh-pressure micro-jet nanometer device, and the nanometer-silver lipidosome can be obtained with the one-step method. The preparing method is simple and easy to operate, the whole production process is environmentally friendly, and the nanometer-silver lipidosome can be industrially produced in a mass mode.

Description

technical field [0001] The invention relates to the technical field of liposomes, in particular to a nano-silver liposome and its preparation method and application. Background technique [0002] When amphiphilic molecules such as phospholipids and sphingolipids are dispersed in the aqueous phase, the hydrophobic tails of the molecules tend to gather together and avoid the aqueous phase, while the hydrophilic heads are exposed to the aqueous phase, forming closed vesicles with a bilayer structure , This is called liposome (liposome). Liposomes are vesicles similar to biomembrane structures, with cell affinity and tissue compatibility, and their diameters are mostly in the range of 25-1000nm. Utilizing the excellent property that liposomes can be fused with cell membranes, it can be widely used in the fields of transgenes and drug carriers, and the research on its performance as a drug carrier and its interaction with cells has also become a hot topic. field of scientific r...

AI Technical Summary

Problems solved by technology

[0005] According to the currently published literature and patents, most nano-silver preparations are obtained by adding strong oxidants such as silver nitrate. Silver nitrate itself has a certain degree of corrosion and toxicity. Under certain circumstances, it will react with microorganisms in the body to generate nitrite. , the latter is highly toxic, and as a preparation for the human body, there is a great potential safety hazard

In clinical practice, nano silver preparations prepared with silver nitrate have a high rate of skin allergy

The nano-silver ions obtained by traditional chemical methods, because of their strong oxidative properties, will have a strong redox reaction with the organism, and can produce a redox reaction with the active tissue of the organism, thereby losing activity and causing damage to the organism. (Traditionally called toxicity), so it is not suitable for human body

In addition, due to its great polarity, it is difficult for nano-silver ions to quickly enter the cytoplasm to function in the face of selectively passing through the cell wall and cell membrane.

[0006] The preparation of nano-silver by inert gas evaporation condensation method and chemical deposition method will inevitably encounter the problem of oxidative denaturation of metal particles because of the need to generate high temperature and use catalysts. The prepared nano-metal materials have uneven particle size distribution and irregular shape. Detection, the particle size distribution is between 20-180nm, and the shape and distribution are not uniform

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