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A kind of anti-mouse RCN3 protein monoclonal scfv antibody and panning method thereof

A monoclonal and antibody technology, applied in the direction of anti-animal/human immunoglobulin, immunoglobulin, chemical instruments and methods, etc., to achieve the effect of wide application prospects

Active Publication Date: 2018-04-13
ZHENGZHOU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no antibody against mouse RCN3 protein that can be purchased and used in China.

Method used

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  • A kind of anti-mouse RCN3 protein monoclonal scfv antibody and panning method thereof
  • A kind of anti-mouse RCN3 protein monoclonal scfv antibody and panning method thereof
  • A kind of anti-mouse RCN3 protein monoclonal scfv antibody and panning method thereof

Examples

Experimental program
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Embodiment Construction

[0025] An anti-mouse RCN3 protein monoclonal ScFv antibody, the base sequence of the monoclonal ScFv antibody is shown in SEQ ID NO: 10, and the amino acid sequence corresponding to the base sequence is shown in SEQ ID NO: 9.

[0026] The monoclonal ScFv antibody includes a light chain variable region V L and heavy chain variable region V H , the light chain variable region V L The amino acid sequence is shown in SEQ ID NO: 7, the heavy chain variable region V H The amino acid sequence of is shown in SEQ ID NO:8.

[0027] The heavy chain variable region contains three hypervariable regions, the amino acid sequences of which are shown in SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3 respectively; the light chain variable region V L It contains three hypervariable regions, the amino acid sequences of which are respectively shown in SEQ ID NO: 4, SEQ ID NO: 5 and SEQ ID NO: 6.

[0028] The monoclonal ScFv antibody includes a full-length antibody formed by ScFv through a disulfi...

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Abstract

The invention belongs to the field of biotechnology, and relates to an antibody and development and production of the antibody, in particular to a monoclonal ScFv antibody phage library for resisting mouse RCN3 protein as well as a selecting method of the monoclonal ScFv antibody. The base sequence of the monoclonal ScFv antibody is shown in SEQ ID NO: 10 and the amino acid sequence corresponding to the base sequence is shown in SEQ ID NO:9. The invention discloses construction and selecting methods of the antibody phage library which has high affinity to mouse RCN3 protein, is displayed on phage surfaces and can be used for efficient screening, sequences of the antibody and eukaryotic expression and antigen detection methods. The ScFv antibody which can realize specific recognition and combines the mouse RCN3 protein molecules can be selected with the selecting method, and the antibody screened by the construction and selecting methods has wide application prospect for detection and disease control related to RCN3 protein.

Description

technical field [0001] The invention belongs to the field of biotechnology, relates to the development and production of an antibody and antibody medicine, in particular to an anti-mouse RCN3 protein monoclonal ScFv antibody and its panning method, the sequence and eukaryotic expression of the anti-mouse RCN3 protein antibody Methods with antigen detection. Background technique [0002] mouse RCN3 It is a single-copy gene with sequence number BC055903, located on chromosome 7 of the mouse, containing 7 exons, encoding 328 amino acids. Studies have shown that the RCN3 protein is located in the endoplasmic reticulum secretion pathway of mice, an endoplasmic reticulum calcium ion-binding protein containing six EF-hand domains, and is associated with the proliferation of lung type I and type II proteins. The gene deletion homozygous mice with artificial knockout of this gene will have shortness of breath and die of purple dryness after delivery. The symptoms are similar to tho...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/18C40B40/06G01N33/577
CPCC07K16/18C07K2317/565C07K2317/622C40B40/06G01N33/577
Inventor 马润林姜远达李闰婷陈龙欣张丽萌兰燕虎李永超
Owner ZHENGZHOU NORMAL UNIV
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