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A kind of cultivation method of nitrosative dominant bacterial group

A technology of dominant flora and cultivation methods, applied in biochemical equipment and methods, using compounds to stimulate growth, microorganisms, etc., can solve the problems of difficult and stable control of DO concentration, ineffective control, long time, etc., to achieve long-term Stabilizing nitrosation ability, shortening culture time, and the effect of strong bacterial tolerance

Active Publication Date: 2020-12-08
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in practical engineering applications, it is difficult to stably control the DO concentration within the required low concentration range. Once the DO concentration is high, the short-cut nitrification will transform into the full-range nitrification
CN1785843A discloses a method for short-range nitrification of wastewater with low C / N ratio and high concentration of ammonia nitrogen. The method uses the nitrification granular sludge cultivated by anaerobic granular sludge as an inoculum, and gradually increases the concentration of ammonia nitrogen in the substrate to realize it. Although oxygen granular sludge has the advantage of fast cultivation speed, in the subsequent aerobic short-cut nitrification process, there are still shortcomings in the transformation from short-cut nitrification to full-range nitrification.
CN102311918A discloses a method for cultivating nitrosative-dominant bacterial flora, which only relies on external conditions for enrichment and cultivation, and the time is relatively long
[0007] At present, short-cut nitrification uses factors such as low dissolved oxygen (DO), high temperature, high pH, ​​and inhibitory factors to control or screen the dominant nitrosative flora. Due to changes in conditions, the phenomenon of conversion from short-cut nitrification to full-range nitrification cannot be effectively controlled.

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  • A kind of cultivation method of nitrosative dominant bacterial group

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Effect test

Embodiment 1

[0032] The first stage: the enrichment of nitrifying bacteria, the initial concentration of ammonia nitrogen in the culture medium is 150mg / L, during the enrichment process, use NaHCO 3 The solution adjusts the pH. Culture conditions: temperature is 24°C, pH is 6.0-7.5, SV30 is 15%-20%, DO is 4mg / L. One cycle per day, the water intake time is 20 minutes, the aeration is 23 hours, the natural settlement is 30 minutes, and the supernatant is removed. Then add the enriched culture solution with the same volume as the supernatant. Every time the culture solution is replaced, the microbial growth promoter A-I must be added according to the concentration of the promoter in the sewage at 20 mg / L. According to this process, the distillation process of GB7479 is used. The concentration of ammonia nitrogen in the water was detected by titration. After no ammonia nitrogen was detected, the concentration of ammonia nitrogen in the pre-added culture solution was increased by 100 mg / L. Aft...

Embodiment 2

[0036] The first stage: the enrichment of nitrifying bacteria, the initial concentration of ammonia nitrogen in the culture medium is 150mg / L, during the enrichment process, use NaHCO 3 The solution adjusts the pH. Culture conditions: temperature is 24°C; pH is 6.0-7.5; SV is 15%-20%; DO is 4mg / L. One cycle per day, the water intake time is 20 minutes, the aeration is 23 hours, the natural settlement is 30 minutes, and the supernatant is removed. Then add the enriched culture solution with the same volume as the supernatant. Every time the culture solution is replaced, the microbial growth promoter A-II must be added according to the concentration of the promoter in the sewage at 25mg / L. According to this process, the distillation method of GB7479 is used. The concentration of ammonia nitrogen in the water was detected by titration method. After no ammonia nitrogen was detected, the concentration of ammonia nitrogen in the pre-added culture solution was increased by 100 mg / L....

Embodiment 3

[0040] The first stage: the enrichment of nitrifying bacteria, the initial concentration of ammonia nitrogen in the culture medium is 150mg / L, during the enrichment process, use NaHCO 3 The solution adjusts the pH. Culture conditions: temperature is 24°C; pH is 6.0-7.5; SV is 15%-20%; DO is 4mg / L. One cycle per day, the water intake time is 20 minutes, the aeration is 23 hours, the natural settlement is 30 minutes, and the supernatant is removed. Then add the enriched culture solution with the same volume as the supernatant, and add the microbial growth promoter A-Ⅲ according to the concentration of the promoter in the sewage at 25 mg / L each time the culture solution is replaced, and operate in a cycle according to this process, using GB 7479 The concentration of ammonia nitrogen in the water was detected by distillation titration. After no ammonia nitrogen was detected, the concentration of ammonia nitrogen in the pre-added culture solution was increased by 100 mg / L. After 1...

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Abstract

The invention relates to a culturing method for a dominant nitrosation bacterial community. The culturing method comprises the following three culturing stages: stage 1, enriching a mixed bacterial community of nitrosomas and nitromonas, wherein a microbial growth promoter A is used in the process of enrichment; stage 2, carrying out nitromonas elutriation by using a microbial growth promoter D and a high-temperature culture and normal-temperature culture alternate method so as to gradually improve the dominance of nitrosomas and beginning third-stage culture when a nitrosation rate is greater than 50%; and stage 3, reducing the content of dissolved oxygen or increasing a pH value, carrying out further nitromonas elutriation and stability domestication of nitrosomas by using the microbial growth promoter D and terminating culture when the nitrosation rate is stabilized and is 65% or more so as to obtain the dominant nitrosation bacterial community. The culturing method provided by the invention overcomes the problems of instability of partial nitrification in practical application, can shorten the starting time of nitrifying process, rapidly changes the process of a nitrifying reaction, broadens the application scope of partial nitrification and provides guarantee for actual application of a partial nitrification process to real projects.

Description

technical field [0001] The invention belongs to the technical field of sewage biological treatment, and in particular relates to a method for cultivating nitrosative dominant bacterial groups. Background technique [0002] The biological denitrification of wastewater mainly relies on the joint action of nitrifying bacteria and denitrifying bacteria in activated sludge, and finally converts various forms of nitrogen in sewage into gaseous nitrogen and escapes from the water. From the perspective of nitrification process, ammonia nitrogen (NH 3 -N) is oxidized to nitrite nitrogen (NO 2 -N) and nitrate nitrogen (NO 3 -N) are two distinct reactions performed by two separate classes of bacteria and should be separable. From the perspective of denitrification process, NO 2 -N and NO 3 -N can be used as the final electron acceptor. Therefore, the whole biological denitrification process can be carried out by NH 3 -N converted to NO 2 -N is converted to N again 2 way to com...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/38C12N1/00
Inventor 高会杰孙丹凤郭志华赵胜楠
Owner CHINA PETROLEUM & CHEM CORP