Two-component polymer microfiber containing synthetic polypeptide and chitosan and its preparation

A technology of ultrafine fiber and chitosan, which is applied in the direction of fiber treatment, fiber chemical characteristics, cellulose/protein conjugated artificial filament, etc., can solve the problem of high cost, improve hydrophilicity, promote adhesion and growth in Proliferation and cost reduction effects

Active Publication Date: 2020-09-22
EAST CHINA UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this type of chemical modification improves the hydrophilicity of the synthesized peptides, the cost is still high

Method used

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  • Two-component polymer microfiber containing synthetic polypeptide and chitosan and its preparation
  • Two-component polymer microfiber containing synthetic polypeptide and chitosan and its preparation
  • Two-component polymer microfiber containing synthetic polypeptide and chitosan and its preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] (1) Preparation of spinning dope

[0040] A mixed solvent was prepared by mixing trifluoroacetic acid and dichloromethane at a volume ratio of 7:3 (v:v). Dissolve poly(γ-benzyl L-glutamate) (molecular weight 220000g / mol) and chitosan (molecular weight 124000g / mol) in the above mixed solvent at a mass ratio of 8:2 (w:w) to prepare A solution with a polymer concentration of 2.0 wt%. The prepared solution was sealed and stirred at room temperature until uniform and transparent to obtain an electrospinning stock solution.

[0041] (2) High voltage electrospinning

[0042] The spinning dope prepared in step (1) was subjected to high-voltage electrospinning under the conditions of spinning voltage 20kV, flow rate 1.0mL / h, and receiving distance 10cm.

[0043] (3) Cross-linking

[0044] Using glutaraldehyde as a cross-linking agent, the chitosan component in the electrospun fiber prepared in step (2) was cross-linked for 12 hours by steam fumigation, and then vacuum-dried ...

Embodiment 2

[0048] The preparation steps are the same as in Example 1, except that the step (3) is not performed, that is, the ultrafine fibers obtained by electrospinning are not cross-linked.

[0049] Fiber morphology was observed by scanning electron microscopy (see figure 2), the fibers were partially damaged after water immersion, indicating that the water resistance of uncrosslinked fibers was worse than that of crosslinked fibers. The cell affinity of fibers was tested by MTT assay (see Figure 4 ), compared with the blank reference, the cell survival rate was 127%, indicating that the prepared uncrosslinked two-component polymer ultrafine fiber cell affinity is weaker than the crosslinked fiber, but it can still significantly promote the growth and proliferation of cells.

Embodiment 3

[0051] (1) Preparation of spinning dope

[0052] Mix dichloroacetic acid and chloroform at a volume ratio of 6:4 (v:v) to prepare a mixed solvent. Dissolve poly(γ-benzyl L-glutamate) (molecular weight: 20,000 g / mol) and chitosan (molecular weight: 300,000 g / mol) in the above mixed solvent at a mass ratio of 9:1, and prepare a polymer concentration of 5.0 wt% solution. The prepared solution was sealed and stirred at room temperature until uniform and transparent to obtain an electrospinning stock solution.

[0053] (2) high voltage electrospinning (same as embodiment 1)

[0054] (3) Cross-linking

[0055] Carry out with reference to embodiment (1) step (3), difference is, the cross-linking agent that adopts is glyoxal.

[0056] (4) fiber performance test (with embodiment 1)

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Abstract

The invention relates to a two-component polymer superfine fiber containing synthetic polypeptide and chitosan and preparation of the two-component polymer superfine fiber. The two-component polymer superfine fiber is prepared through the following steps: (1) preparation of spinning liquid: a mixed solvent containing volatile organic acid and volatile halogenated hydrocarbon is prepared, then hydrophobic synthetic polypeptide and chitosan are dissolved in the mixed solvent and stirred until the liquid is uniform and transparent, and the spinning liquid is obtained; (2) high-voltage electrospinning: the spinning liquid obtained in the step (1) is subjected to high-voltage electrospinning, and the two-component polymer superfine fiber containing the synthetic polypeptide and the chitosan is prepared. Compared with the prior art, the synthetic polypeptide and the chitosan both have good biocompatibility and biodegradability, so that the cost of the synthetic polypeptide fiber can be reduced after the synthetic polypeptide and the chitosan are blended, and actual application of the fiber in the fields of tissue engineering scaffolds, medical dressings, drug sustained release and the like is facilitated.

Description

technical field [0001] The invention relates to the technical field of preparation of functional ultrafine fibers, in particular to a two-component polymer ultrafine fiber containing synthetic polypeptide and chitosan and a preparation method thereof. Background technique [0002] Electrospinning is a method in which polymer solution or melt is sprayed and stretched under high-voltage electrostatic action to obtain ultrafine fibers, and it has become one of the main methods for preparing micron or nanometer fibers. [0003] Since the synthetic polypeptide has the same main chain structure and secondary structure as the natural protein, and has good biocompatibility, degradability, and is easily absorbed and metabolized by the body, it has a good application prospect in the field of biomedical materials. . In addition, the molecular chains of synthetic peptides often adopt rigid conformations such as α-helices or β-sheets, and the molecular chains have a large cumulative dip...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): D01F8/02D01F8/18D01D5/00D06M13/123D06M13/127
CPCD01D5/003D01F8/02D01F8/18D06M13/123D06M13/127
Inventor 赵黎明陈涛王乐军邱勇隽魏杰路亚亮黄文景
Owner EAST CHINA UNIV OF SCI & TECH
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