Differentially expressed proteins in brain tissues of mice infected with toxoplasma gondii, protein interacting with developmentally regulated brain protein and application of protein

Abstract

The invention provides a method for studying differentially expressed proteome in brain tissues of mice chronically infected with toxoplasma gondii by an iTRAQ (Isobaric Tags For Relative And Absolute Quantitation) technology. The invention also provides a protein or polypeptide interacting with developmentally regulated brain protein and application of the protein or polypeptide. In the invention, six toxoplasma gondii proteins interacting with the developmentally regulated brain protein are found by CoIP and Pulldown experiments; and gene knockout experiments and neurobehavioral validation are further performed on TgRPS14 gene and TgH4 gene. The method for studying differentially expressed proteome in brain tissues of mice chronically infected with toxoplasma gondii provided by the invention can rapidly and effectively perform proteomics research, and is beneficial for rapidly positioning to detection targets and prevention and treatment medicine target points with a research value or a commercial application value.

Description

[0001] This application claims the Chinese patent application number 201710162190.0 submitted to the Chinese Patent Office on March 17, 2017, and the title of the invention is "Proteins differentially expressed in brain tissues of mice infected with Toxoplasma gondii, proteins interacting with brain development regulatory proteins and their applications" priority of the patent application, the entire content of which is incorporated in this application by reference. technical field [0002] The invention relates to the field of parasitology, and relates to a protein differentially expressed in the brain tissue of mice infected with Toxoplasma gondii, a protein interacting with a brain development regulatory protein and its application; specifically, it relates to a method of using iTRAQ technology to study the brain of mice chronically infected by Toxoplasma gondii The method for organizing differentially expressed proteomes also relates to a protein or polypeptide interacting ...

AI Technical Summary

Problems solved by technology

[0006] There is no report on the method of using iTRAQ technology to study the differentially expressed proteome in the brain tissue of mice chronically infected with Toxoplasma gondii

[0007] In addition, after obtaining the differentially expressed proteome in the brain tissue of mice chronically infected with Toxoplasma gondii, it is necessary to conduct functional analysis and functional verification of the differentially expressed proteins in order to dig out detection targets and drug targets for prevention and treatment; currently, due to the effective acquisition of small There is a lack of methods for differentially expressing proteomes in mouse brain tissue, which makes it difficult to advance research on differentially expressed protein-interacting proteins in mouse brain tissue chronically infected with Toxoplasma gondii, which is not conducive to the discovery and application of detection targets and prevention and treatment drug targets

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