Z-type simple parallel plate flow chamber

Abstract

The invention provides a Z-shaped simple parallel plate flow chamber, which includes a bottom plate, a cover plate and a glass slide. The bottom plate is provided with a glass slide slot and an observation hole, and the cover plate is provided with an entrance and exit and an entrance and exit buffer groove. The level of the buffer platform at the entrance and exit of the slide is higher than the effective area of ​​the slide, and the entrance and exit of the cover plate are perpendicular to the groove surface of the entrance and exit buffer tank, so that the fluid pressure will not directly act on the entrance and exit structure. The flow in the effective action area of ​​the slide is stable, the effective action area is defined, and the effective action area ratio is improved. The fluid shear stress received by the cells in the effective action area of ​​the slide is very uniform, which weakens the inlet and outlet effects of the parallel plate flow chamber. It can meet the needs of cell biomechanical experimental research, and by adjusting the inlet flow, the fluid shear stress can be regulated.

Description

technical field [0001] The invention belongs to the field of cell biomechanics research, and in particular relates to a mechanical loading experiment device for in vitro culture of isolated cells. Background technique [0002] Due to the complexity of the physiological structure of biological tissues and organs and the diversity of physiological environments in living organisms, the physiological and mechanical environments in which somatic cells live are also intricate. With the development of in vitro culture and isolation technology of cells, it has become an effective and popular experimental method to conduct cell-level experiments under controlled conditions in vitro. The simple parallel plate flow chamber is an in vitro cell culture device that uses the flow of culture liquid to generate shear stress to act on the cell surface. It is used to study the changes in the shape, adhesion and function of cells under different shear forces. It is one of the main experimental...

AI Technical Summary

Problems solved by technology

The position of the shear stress field in the existing simple parallel plate flow chamber is unclear and cannot provide a high enough effective area ratio characteristic, resulting in the failure or failure of many cell biomechanical experiments

[0003] The Chinese patent application number 201420372524.9 discloses a parallel plate flow chamber device with a replaceable inner core. The design of the bottom plate structure of the device is relatively complicated, resulting in high manufacturing costs and a small uniform shear stress field.

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