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Chiral analysis convergence chromatography tandem mass spectrometry for nicotine in tomato

A technology of convergence chromatography and tandem mass spectrometry, applied in the field of chemical composition determination of tomatoes, can solve the problems such as no method for chiral analysis of nicotine in tomatoes, complicated processing steps, inability to meet the detection of a large number of samples, etc. High sensitivity and avoid false positive effect

Active Publication Date: 2017-06-09
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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Problems solved by technology

[0003] At present, the chiral analysis of nicotine is mainly studied by gas chromatography. For example, the patent application number 201610663534.1 "A Method for Chiral Analysis of Nicotine in Cigarette Tobacco" has complicated pretreatment steps and a long detection time. Up to 140min, which cannot meet the detection of a large number of samples. At present, there is no method for chiral analysis of nicotine in tomatoes

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  • Chiral analysis convergence chromatography tandem mass spectrometry for nicotine in tomato
  • Chiral analysis convergence chromatography tandem mass spectrometry for nicotine in tomato
  • Chiral analysis convergence chromatography tandem mass spectrometry for nicotine in tomato

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Embodiment Construction

[0015] The present invention is further described by the following specific examples, but does not limit the present invention.

[0016] 1. Instruments and Reagents

[0017] ACQUITY UPC2 ultra-high performance convergence chromatograph (Waters, USA), 6410B triple quadrupole mass spectrometer (Agilent, USA), AE163 electronic balance (sensitivity: 0.0001 g, Mettler, Switzerland), high-speed pulverizer (Wuhan Yincai Technology Co., Ltd.), ColeparmerVotex-Genie vortex oscillator in the United States, and SIGMA 3-30K-high-speed desktop refrigerated centrifuge in Germany. Methyl tert-butyl ether (chromatographically pure), sodium hydroxide (analytical pure), formic acid (chromatographically pure), methanol (chromatographically pure), S-(-)-nicotine standard (CAS: 54-11-5) , R-(+)-nicotine standard (CAS: 25162-00-9).

[0018] 2. Preparation of standard solution

[0019] Weigh about 100.0 mg of S-(-)-nicotine standard substance, dilute it with methanol solution to a 50 mL brown vol...

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Abstract

The invention discloses a chiral analysis convergence chromatography tandem mass spectrometry for nicotine in tomato. The chiral analysis convergence chromatography tandem mass spectrometry is characterized by comprising the following steps: drying, smashing and sieving tomato samples, after soaking the samples with a sodium hydroxide solution, adopting methyl tertiary butyl ether and a methanol solution for extraction, after matrix dispersive solid-phase extraction of an extract liquor, using two chiral columns to carry out tandem-convergence chromatography tandem mass spectrometry analysis, and adopting a peak area normalization method to quantitatively detect the proportions of S-(-)-nicotine and R-(+)-nicotine in the tomato. According to the detection method provided by the invention, the sample extraction purification method is simple and efficient, the pollution of the sample liquor to chromatographic columns can be reduced, the convergence chromatography tandem mass spectrometry detection method is high in sensitivity and high in specificity, false positive can be eliminated, the analysis time is short, and chiral analysis of the nicotine in a large batch of tomato samples can be met.

Description

technical field [0001] The invention relates to a method for determining chemical components of tomatoes, in particular to a chiral analysis convergence chromatography tandem mass spectrometry method for nicotine in tomatoes. Background technique [0002] Nicotine, also called nicotine, is an alkaloid found in plants of the nightshade family and an important component of tobacco. The nicotine molecule contains a chiral center, which is the 2-position carbon atom on the tetrahydropyrrole ring, so nicotine has two enantiomers: S-(-)-nicotine and R-(+)-nicotine. The two enantiomers of nicotine have completely different contents, metabolic mechanisms and physiological properties. Therefore, chiral analysis of nicotine in tomato is required. [0003] At present, the chiral analysis of nicotine is mainly studied by gas chromatography. For example, the patent application number 201610663534.1 "A Method for Chiral Analysis of Nicotine in Cigarette Tobacco" has complicated pretreat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 张洪非费婷罗彦波陈欢李翔宇姜兴益庞永强吴达戚大伟朱风鹏胡少东付亚宁韩书磊
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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