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Breeding method for eriosoma lanigerum

A technology of apple cotton aphid and apple, which is applied in animal husbandry and other fields, can solve the problems of not being able to meet the needs of experiments, unable to continuously raise a large number of apple cotton aphids, and low success rate of inoculation of apple cotton aphid, so as to improve the success rate of inoculation and low hardness , loose texture effect

Inactive Publication Date: 2017-06-13
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Apple cotton aphid mainly reproduces viviparously in the form of parthenogenesis in China. In the past, apple cotton aphid was mainly bred with 1-year-old crabapple seedlings through cultivation management and inoculation methods. Since crabapple trees are relatively resistant to aphids, apple cotton aphid The success rate of inoculation is low. Therefore, this method cannot continuously raise a large number of apple aphids, and cannot meet the needs of the experiment.

Method used

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  • Breeding method for eriosoma lanigerum
  • Breeding method for eriosoma lanigerum
  • Breeding method for eriosoma lanigerum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1. The source of tested insects:

[0017] The apple aphid was collected from the poorly managed apple orchard in Chengyang District, Qingdao City, Shandong Province.

[0018] 2. Instruments and materials:

[0019] Fruit ring cutting knife, incubator, label paper, tweezers, beaker, wool, brush, pick needle, absorbent cotton, Nongfu Spring 600mL empty bottle, etc.

[0020] 3. Method steps

[0021] (1) Selection of trees for inoculation: At the beginning of June, red Fuji apple seedlings grafted with two-year-old Begonia spp. were selected for inoculation.

[0022] (2) Handling of branches: on the branches of Red Fuji, 3 different ways of processing the branches are adopted: horizontal knife-cutting method, vertical knife-cutting method, and blank control;

[0023] Table 1 compares the treatment methods of apple branches

[0024]

[0025] (3) Collect the source of apple aphids: during the period of May-June when apple aphids are harmful, choose apple trees that are ...

Embodiment 2

[0046] 1. Source of tested insects: the same as in Example 1.

[0047] 2, apparatus and material: with embodiment 1.

[0048] 3. Method steps

[0049] (1) Selection of seedlings for inoculation: At the beginning of June, we selected Red Fuji apple seedlings grafted with two-year-old Begonias, New Red Star apple seedlings grafted with two-year-old Begonias, and Jonakin grafted with two-year-old Begonias. Apple seedlings and biennial octagonal begonia seedlings.

[0050] (2) Treatment of branches: The longitudinal knife-cutting method was used to process the branches on four kinds of inoculated seedlings.

[0051]Table 4: Comparison of treatment methods for apple branches

[0052] method specific operation Numbering Red Fuji Apple Seedlings Longitudinal knife cut F New Red Star Apple Seedlings Longitudinal knife cut G Jonah Gold Apple Seedlings Longitudinal knife cut H Begonia seedlings Longitudinal knife cut I

[0053] (3) Co...

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Abstract

The invention discloses a breeding method for eriosoma lanigerum. A lot of standard test insects consistent in dimension are provided for research on eriosoma lanigerum. The method includes the steps that trees for inoculation are selected, the seed source of the eriosoma lanigerum is collected, primary treatment is carried out on the eriosoma lanigerum, branches are longitudinally cut and treated, the eriosoma lanigerum is inoculated, bottle sleeving treatment is carried out, and aphids of the eriosoma lanigerum are formed and propagated. In the method, red fuji apple seedlings are used for replacing cherry-apple tress to serve as propagation hosts for eriosoma lanigerum, and therefore the success rate of inoculation is greatly increased; by the adoption of a longitudinal branch cutting method, attaching and parasitizing are convenient for the eriosoma lanigerum, callus tissue is conveniently formed, the eriosoma lanigerum can easily absorb nutritive substances, and it is avoided that by the adoption of a transverse cutting method, sieve tubes are cut off, so that the eriosoma lanigerum has no way to absorb sufficient nutritive substances; bottle sleeving is carried out, water is dropwise added to cotton regularly, in this way, small environment and high humidity of the bred eriosoma lanigerum can be kept, natural conditions of the eriosoma lanigerum in field outbreak are met, and parasitizing of woolly aphid parasites as natural enemies can be stopped for the eriosoma lanigerum.

Description

technical field [0001] The invention belongs to the technical field of insect feeding, and in particular relates to a feeding method of the apple cotton aphid. Background technique [0002] The apple aphid (Eriosoma lanigerum (Hausmann)) belongs to Hemiptera, Pemphigidae, and Eriosoma Leech. It is also known as blood-colored aphid, red aphid, and cotton aphid. One of the important quarantine pests of the genus Malus. The apple aphid can parasitize and suck sap on the ground and underground parts of apple trees all year round. At the same time, it can secrete salivary digestive enzymes, stimulate the proliferation of parasitic tissues and form tumors, and affect nutrient transport. Apple cotton aphid can secrete white waxy cotton flocs on the body surface and excrete honeydew. The main components of honeydew are amino acids and sugars. ) ideal culture medium, fumigatus can cause the tree body and leaves to turn black, reduce the photosynthesis of leaves, and form rust and s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 周洪旭刘绪坤谭秀梅杨勤民万方浩
Owner QINGDAO AGRI UNIV
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