Application of propolis ethanol extract

An ethanol extraction and extract technology, applied in the field of medicine, can solve problems such as the drug application of ulcerative colitis of propolis extract that has not been seen

Inactive Publication Date: 2017-06-20
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the prior art, the use of propolis in the preparation of medicines for the treatment of various diseases basically uses the compatibility compone

Method used

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  • Application of propolis ethanol extract
  • Application of propolis ethanol extract
  • Application of propolis ethanol extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034]The preparation method of propolis ethanol extract: Weigh 100g propolis, use a grinder to grind into powder, extract propolis sample with 1L 95% (v / v) ethanol, and ultrasonicate at 40°C for 3h (frequency 600KHz, power 600W). The supernatant after ultrasonication was filtered with No. 4 filter paper to remove residual impurities. Re-infiltrate the residual impurities with 95% (v / v) ethanol and sonicate for 3 hours, collect the filtered supernatant, repeat the above operation, and extract a total of 3 times. Afterwards, all the filtrates were collected centrally, and evaporated and recovered using a 50° C. reduced-pressure rotary evaporator. Finally, the propolis ethanol extract is obtained by drying in an oven until the extract has a constant weight, and the extract is stored in a -20°C refrigerator until use.

Embodiment 2

[0036] (1) The experimental animals were male SPF grade SD rats (45, 8 weeks old, weighing 190±20 g) purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd. The animals were raised in the Experimental Animal Center of Zhejiang Academy of Traditional Chinese Medicine, and the culture environment was 12h light + 12h dark, the temperature was 23°C, the relative humidity was 50%-70%, and they had free access to food and water.

[0037] (2) After a week of adaptive feeding, the rats were randomly divided into 5 groups: blank control group (n=5); model group (n=10); propolis low-dose group (n=10, supplemented with 0.1% propolis); propolis medium dose group (n=10, 0.2% propolis added to the diet); propolis high dose group (n=10, 0.3% propolis added to the diet). The dietary formula refers to the American AIN-93M standard formula (Table 1).

[0038] Table 1: Animal Diet Formulations

[0039]

[0040] (3) After the experimental rats were fed with the experime...

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Abstract

The invention relates to the technical field of pharmaceuticals, and more particularly to application of a propolis ethanol extract in preparation of medicaments, food and health care products for prevention or treatment of inflammatory bowel diseases. The propolis ethanol extract is obtained by extraction by using 80 to 95% ethanol as a solvent. Research findings show that the propolis ethanol extract can inhibit the health state of a body during the development course of ulcerative colitis, reduce the shortening of the length of the colon, and improve the pathological structure of a colon tissue; therefore, the propolis ethanol extract can be applied to development of medicaments, health care products and food for prevention or treatment of the ulcerative colitis.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of propolis ethanol extract. Background technique [0002] Inflammatory bowel disease (IBD) is a group of diseases characterized by intestinal inflammation, mainly including Crohn's disease (CD) and ulcerative colitis (UC). The pathogenesis of inflammatory bowel disease is not yet fully clarified. Clinically, inflammatory bowel disease often manifests as recurrent attacks and is very difficult to cure. It is generally believed that its pathogenesis is closely related to the complex interaction between host factors and external factors. The main factors leading to the onset of inflammatory bowel disease include intestinal microenvironment disorder, immune disorder, changes in the external environment, and genetic factors of the host. According to statistics, there are about 5 million patients with inflammatory bowel disease in the world, including about 1.4 mill...

Claims

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Application Information

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IPC IPC(8): A61K35/644A61P1/00A61P1/04A23L33/10A23L21/20A21D2/34
CPCA61K35/644A21D2/34A23V2002/00A23V2200/32
Inventor 王凯胡福良吴黎明薛晓锋
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
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