Unlock instant, AI-driven research and patent intelligence for your innovation.

The application and kit of the combination of proheme and β-glucuronidase in the detection of heterogeneous hyperplasia of cervical epithelial cells

A cervical epithelial cell and detection kit technology, applied in the field of liquid biopsy pathological detection, can solve the problems of low sensitivity and specificity of detection results, inaccurate diagnosis results of cervical cancer, etc., and achieve the effect of improving accuracy

Inactive Publication Date: 2019-03-22
山东瓦博格生物技术有限公司
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the current clinical screening methods for detecting cervical epithelial cell heterogeneity are all based on the detection of a single marker, the sensitivity and specificity of the detection results are not high, and the diagnostic results of cervical cancer are not accurate

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • The application and kit of the combination of proheme and β-glucuronidase in the detection of heterogeneous hyperplasia of cervical epithelial cells
  • The application and kit of the combination of proheme and β-glucuronidase in the detection of heterogeneous hyperplasia of cervical epithelial cells
  • The application and kit of the combination of proheme and β-glucuronidase in the detection of heterogeneous hyperplasia of cervical epithelial cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] This embodiment provides a kit for detecting cervical epithelial cell heterogeneous hyperplasia, which uses proheme and β-glucuronidase as markers for combined detection. Through the test results of proheme and β-glucuronidase, it is used to determine the heterogeneous hyperplasia of cervical epithelial cells (such as heterogeneous hyperplasia of cervical squamous epithelial cells or cervical columnar epithelial cells) Happening.

[0062] The cervical epithelial cell heterogeneous hyperplasia detection kit provided in this embodiment includes a proheme detection reagent membrane for detecting proheme and β-glucuronidase for detecting β-glucuronidase Test reagent film.

[0063] Among them, the protohemoglobin detection reagent membrane provided by the present invention is made as follows:

[0064] 1.1. Make phosphate buffer. The buffer is 1.75mol / L, pH 4.5 phosphate buffer.

[0065] 1.2. Add a quantitative amount of polyvinylpyrrolidone K30 to the above-mentioned phosphate bu...

Embodiment 2

[0096] This embodiment provides a kit for detecting cervical epithelial cell heterogeneous hyperplasia, which uses proheme and β-glucuronidase as markers for combined detection. The test results of proheme and β-glucuronidase are used to determine the heterogeneous cell proliferation of the sample.

[0097] The cervical epithelial cell heterogeneous hyperplasia detection kit provided in this embodiment includes a proheme detection reagent membrane for detecting proheme and β-glucuronidase for detecting β-glucuronidase Detection reagent film and sample pretreatment liquid used to process the sample.

[0098] Among them, the preparation method of the proheme detection reagent membrane, the β-glucuronidase detection reagent membrane and the sample pretreatment liquid can refer to Embodiment 1.

[0099] The effect of the cervical epithelial cell heterogeneous hyperplasia detection kit provided in this example is the same as that of Example 1.

Embodiment 3

[0101] This example provides verification of the sensitivity, specificity, and Youden index of using protohemoglobin and β-glucuronidase as a combined marker to detect the heterogeneous hyperplasia of cervical epithelial cells.

[0102] Sensitivity: also known as sensitivity, refers to the proportion of screening methods that can correctly determine the actual patients as patients; specificity: refers to the screening methods that can accurately correct people The proportion of non-patients.

[0103] 3.1 Sensitivity verification

[0104] 3.1.1 Specimen source: 100 samples of mixed liquid composed of secretion, tissue exudate and mucus of cervical cancer patient’s cervix contained in the clinical laboratory of a medical unit. Cervical cancer was diagnosed by pathological examination.

[0105] 3.1.2 Detection method: using the cervical epithelial cell heterogeneous hyperplasia detection kit provided in Example 2 to perform the combined detection of proheme and β-glucuronidase on the ab...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
concentrationaaaaaaaaaa
Login to View More

Abstract

The invention discloses application of a heme and beta-glucuronidase union in heterogeneity hyperplasia detection of cervical epithelial cells and a kit, and belongs to the technical field of fluid pathological biopsy. Heme and beta-glucuronidase are applied in the field of heterogeneity hyperplasia detection of cervical epithelial cells for the first time as a union marker, and surprisingly, compared with the detection separately using heme or beta-glucuronidase as a marker, the sensitivity, specificity and Youden index of detection using heme and beta-glucuronidase as a union marker are all obviously improved. Thus, using of heme and beta-glucuronidase as a union marker is beneficial to improving the accuracy of diagnosis results of cervical cancers caused by heterogeneity hyperplasia of cervical epithelial cells.

Description

Technical field [0001] The present invention relates to the technical field of liquid biopsy pathological detection, in particular to the application and kit of proheme and β-glucuronidase in the detection of cervical epithelial cell heterogeneous hyperplasia. Background technique [0002] The result of heterogeneous proliferation of cervical epithelial cells is cervical cancer. [0003] Cervical cancer is the second leading cancer killer for women in the world. The incidence of cervical cancer in my country is 6 times that of developed countries, and it is showing a younger trend. There are about 150,000 new cases of cervical cancer in my country every year, accounting for about 1 / 3 of the total number of patients worldwide, and nearly 80,000 women die from this. This shows that cervical cancer has become one of the killers of Chinese women, destroying the health of tens of thousands of women, and affecting the happy lives of millions of families in China. In recent years, the i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/34G01N33/574
CPCG01N33/57442
Inventor 温鹏
Owner 山东瓦博格生物技术有限公司