Preparation method and application of geranium wilfordii polysaccharide and oligosaccharide

A technology of geranium and oligosaccharides, which is applied in the field of medicine, can solve problems that have not been reported, and achieve the effects of wide application range, enhanced immunity, and easy operation

Inactive Publication Date: 2017-06-23
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current research on its active ingredients mainly involves tannins, flavonoids, organic acids, volatile oils, triterpenoids, sterols, lignin and other components. The research on polysaccharides and oligosaccharides therein has not been reported, especially how to solve The integrated preparation of geranium polysaccharides and oligosaccharides has not been reported

Method used

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  • Preparation method and application of geranium wilfordii polysaccharide and oligosaccharide
  • Preparation method and application of geranium wilfordii polysaccharide and oligosaccharide
  • Preparation method and application of geranium wilfordii polysaccharide and oligosaccharide

Examples

Experimental program
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Effect test

Embodiment 1

[0010] In the specific implementation of the present invention, the geranium is dried and crushed into coarse powder; the geranium coarse powder is taken, and distilled water is added to the geranium coarse powder, and the weight ratio of the geranium coarse powder to distilled water is 1:22, Soak for 46 minutes, ultrasonically extract at 98°C for 97 minutes, filter, concentrate the filtrate to an extract with a specific gravity of 1.20, add ethanol to make the ethanol content reach a volume concentration of 79%, let stand at 4°C for 15 hours, and centrifuge at 6000r / min for 45 minutes to obtain supernatant A and centrifuged precipitate B, supernatant A for later use, centrifuged precipitate B freeze-dried to obtain geranium crude polysaccharide powder C, take geranium crude polysaccharide powder C and add 10 times the weight of distilled water to dissolve to obtain geranium crude polysaccharide solution D , add 1% by weight of yeast to the geranium crude polysaccharide solutio...

Embodiment 2

[0012] In the present invention, the geranium is dried and crushed into coarse powder; the geranium coarse powder is taken, and distilled water is added to the geranium coarse powder, and the weight ratio of the geranium coarse powder to distilled water is 1:11, soaked for 23 minutes, Ultrasonic extraction at 84°C for 41min, filter, concentrate the filtrate to an extract with a specific gravity of 1.11, add ethanol to make the ethanol content reach a volume concentration of 66%, let it stand at 4°C for 8h, and centrifuge at 6000r / min for 45min to obtain supernatant A and centrifugal precipitate B, the supernatant A is set aside, and the centrifugal precipitation B is freeze-dried to obtain the geranium crude polysaccharide powder C. Take the geranium crude polysaccharide powder C and add 10 times the weight of distilled water to dissolve it to obtain the geranium crude polysaccharide solution D. Add 1% of its weight yeast to the stork grass crude polysaccharide solution D, cult...

Embodiment 3

[0014] Take geranium and dry it, crush it into coarse powder; take geranium coarse powder, add distilled water to the geranium coarse powder, the weight ratio of geranium coarse powder to distilled water is 1:16, soak for 35min, 91 ℃ ultrasonic extraction 69min, filter, concentrate the filtrate to an extract with a specific gravity of 1.15, add ethanol to make the ethanol content reach a volume concentration of 72.5%, let stand at 4°C for 11h, and centrifuge at 6000r / min for 45min to obtain supernatant A and centrifugal precipitate B, supernatant Liquid A is set aside, centrifuged and precipitated B is freeze-dried to obtain geranium crude polysaccharide powder C, take geranium crude polysaccharide powder C and add 10 times the weight of distilled water to dissolve the geranium crude polysaccharide solution D, and add geranium crude polysaccharide Add 1% of its weight yeast to solution D, cultivate and ferment on a shaker with a rotating speed of 173r / min at room temperature, a...

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Abstract

The invention relates to a preparation method and application of geranium wilfordii polysaccharide and oligosaccharide. The problem about integrated preparation of the geranium wilfordii polysaccharide and oligosaccharide can be effectively solved by applying an ultrasonic extracting technology and a fermenting technology to extracting and purifying processes of the geranium wilfordii polysaccharide and oligosaccharide; the geranium wilfordii polysaccharide prepared by the preparation method can be effectively used for preparing immunity enhancing medicaments and healthcare products; the geranium wilfordii oligosaccharide prepared by the preparation method can be effectively used for preparing anti-fatigue medicaments and healthcare products.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a preparation method and application of geranium polysaccharides and oligosaccharides. Background technique [0002] Geranium is the dry aerial part of Erodium stephanianum Willd., Geranium wilfordii Maxim. or Geranium carolinianum L. of Geranium family. It has the functions of dispelling rheumatism, dredging meridians, stopping diarrhea and dysentery, antibacterial, antiviral, antioxidant, antitumor, anti-inflammatory and analgesic, hypoglycemic, and liver protection. Clinically, it is mainly used for rheumatic arthralgia, numbness and spasm, muscle and bone pain, and diarrhea and dysentery. The current research on its active ingredients mainly involves tannins, flavonoids, organic acids, volatile oils, triterpenoids, sterols, lignin and other components. The research on polysaccharides and oligosaccharides therein has not been reported, especially how to solve The integrated preparati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C07H3/06C07H1/08A61K31/715A61K31/702A61P37/04A61P39/00
Inventor 张明昊刘富岗李玉洁
Owner HENAN UNIV OF CHINESE MEDICINE
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