A kind of L-theanine iron chelate and its preparation method and instant tea powder containing the chelate and its preparation method

A technology of iron theanine and instant tea powder, applied in the fields of instant tea powder and preparation, L-theanine iron chelate and preparation, can solve the problem that L-theanine and ferrous ions have no relevant reports, electrolysis The synthesis method is expensive and time-consuming, and has problems such as adverse gastrointestinal reactions, and achieves the effect of improving bioavailability, improving immunity, and excellent taste.

Active Publication Date: 2020-08-04
SHAANXI NORMAL UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The first generation product is unstable, easy to deliquescence, easy to oxidize, and has iron smell
The absorption and utilization rate of the second-generation iron supplement has been improved, but it will cause gastrointestinal adverse reactions
The third-generation product has stable properties, good taste, high utilization rate, and does not irritate the stomach, but the production cost is high
Electrosynthesis is expensive and time-consuming
At present, there are many researches at home and abroad on ferrous glycine, ferrous methionine, ferrous tyrosine, etc., but there are almost no related reports on the chelate of L-theanine and ferrous ion

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of L-theanine iron chelate and its preparation method and instant tea powder containing the chelate and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A method for preparing instant tea powder containing L-theanine iron chelate, comprising the steps of:

[0036] First: inoculate Bacillus subtilis stored on a slant at 4°C on a plate medium, culture at 37°C for 16 hours, and then transfer to 3mL liquid medium for activation. The secondary seed liquid of Bacillus subtilis activated twice was transferred to 30 mL of fermentation culture liquid according to the volume fraction of 3% inoculum, placed in a constant temperature incubator for shaking culture at 37°C, and cultured overnight for 12 hours.

[0037]Second: collect the fermented liquid and centrifuge at 8000 rpm for 25 minutes to obtain crude enzyme liquid of γ-glutamyl transpeptidase. Enzyme activity was measured using a spectrophotometer. Select 20 mL of crude enzyme solution with an enzyme activity of 0.01 U / mL for the catalytic reaction.

[0038] The 3rd: add respectively the L-glutamine (concentration 15mmol / L) of crude enzyme liquid weight 10% and ethylamin...

Embodiment 2

[0045] A method for preparing instant tea powder containing L-theanine iron chelate, comprising the steps of:

[0046] First: Inoculate Bacillus subtilis stored on a slant at 4°C on a plate medium, culture at 37°C for 16 hours, and then transfer to 3mL liquid medium for activation. The secondary seed liquid of Bacillus subtilis activated twice was transferred to 30 mL fermentation culture liquid according to the volume fraction of 3% inoculum, placed in a constant temperature incubator for shaking culture at 37°C, and cultured overnight for 16 hours.

[0047] Second: collect the fermented liquid and centrifuge at 8000 rpm for 25 minutes to obtain crude enzyme liquid of γ-glutamyl transpeptidase. Enzyme activity was measured using a spectrophotometer. Select 20 mL of crude enzyme solution with an enzyme activity of 0.15 U / mL for the catalytic reaction.

[0048] The 3rd: add respectively the L-glutamine (concentration 30mmol / L) of crude enzyme liquid weight 10% and ethylamine ...

Embodiment 3

[0055] A method for preparing instant tea powder containing L-theanine iron chelate, comprising the steps of:

[0056] First: Inoculate Bacillus subtilis stored on a slant at 4°C on a plate medium, culture at 37°C for 16 hours, and then transfer to 3mL liquid medium for activation. The secondary seed liquid of Bacillus subtilis activated twice was transferred to 30 mL of fermentation culture liquid according to the volume fraction of 3% inoculum, placed in a constant temperature incubator for shaking culture at 37°C, and cultured overnight for 14 hours.

[0057] Second: collect the fermented liquid and centrifuge at 8000 rpm for 25 minutes to obtain crude enzyme liquid of γ-glutamyl transpeptidase. Enzyme activity was measured using a spectrophotometer. Select 20 mL of crude enzyme solution with an enzyme activity of 0.2 U / mL for the catalytic reaction.

[0058] The 3rd: add respectively the L-glutamine (concentration 50mmol / L) of crude enzyme liquid weight 10% and ethylamin...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to an L-theanine iron chelate and a preparation method thereof and instant tea powder containing the chelate and a preparation method thereof. The preparation method of the L-theanine iron chelate comprises the following steps of: by taking L-theanine and ferrite as raw materials, forming a reaction system by taking a buffer liquid, the pH value of which is 3-7, as a reaction medium; mixing the mixture at 30-80 DEG C; and after reaction, separating and purifying a reaction liquid to obtain the L-theanine iron chelate, wherein the mass ratio of L-theanine to ferrite is (4-1): 1, and the L-theanine is prepared from gamma-glutamyltranspeptidase fermented by bacillus subtilis by taking L-glutamine and ethylamine hydrochloride as primers. By preparing the L-theanine by adopting a microbial enzyme method, the L-theanine iron chelate is obtained by adopting chelated ferrous ions and L-theanine by means of a water system synthetic method on the basis. The L-theanine iron chelate is low in production cost, high in environmental safety and low in resource consumption, and massive safe production can be performed.

Description

technical field [0001] The invention relates to a theanine chelate and a preparation method of a functional tea beverage thereof, in particular to an L-theanine iron chelate and a preparation method thereof, and instant tea powder containing the chelate and a preparation method thereof. Background technique [0002] Iron is an essential trace element in the human body. Iron participates in the normal transport of oxygen in the tissues of the body, directly affects the energy and protein metabolism of the body, and also affects the immune function of the body. According to the survey, 67.5% of pregnant women in China will suffer from anemia. If pregnant women are anemic, they will experience symptoms such as fatigue, fatigue, dizziness, tinnitus, and inattention. Anemia in pregnant women is very harmful to pregnant women and fetuses. Severe anemia can lead to fetal hypoxia, intrauterine growth retardation, premature birth and even death. Iron is absorbed as ferrous iron (fe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C07C231/12A23L33/165A23L2/39
CPCA23L2/39A23V2002/00A23V2200/30A23V2200/324
Inventor 陈立李建科
Owner SHAANXI NORMAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap