Basic group editing system as well as constructing and applying methods thereof

A base editing and base technology, applied in the field of genome editing, can solve problems such as reducing the efficiency of base editing, and achieve the effect of enhancing the efficiency, reducing the rate of base insertion or deletion, and improving the rate of C-T base editing.
CN106916852AActive Publication Date: 2017-07-04SHANGHAI TECH UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHANGHAI TECH UNIV
Publication Date
2017-07-04

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Abstract

The invention provides a basic group editing system as well as constructing and applying methods of the basic group editing system. The basic group editing system is characterized y comprising a UGI expression vector and a BE3 expression vector, or a UGI and BE3 coexpression vector. According to the basic group editing system as well as the constructing and applying methods of the basic group editing system, for the first time, the C-T editing ratio generated by a CRISPR basic group editor is increased and the basic group insertion or loss ratio caused by the CRISPR system is reduced internationally, then the efficacies of the CRISPR basic group editor is enhanced, and thus a novel method and a novel thought are provided for the implementation of the accurate and safe basic group editing of the CRISPR basic group editor in the genomes of the various species.
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Description

technical field

[0001] The invention relates to the field of genome editing, in particular to an enhanced CRISPR base editing system to realize high-efficiency and high-precision base-level genome editing methods and applications thereof. Background technique

[0002] Genome editing technology refers to a genetic engineering technology that uses designable nucleases (molecular scissors) to modify specific segments of the genome DNA of an organism through base insertion, deletion or substitution to achieve editing of the target gene. Using genome editing technology to genetically manipulate cells can be widely used in basic life science research, biotechnology development, agricultural technology development, and pharmaceutical research and development. For example: directly correcting the genetic mutations that cause genetic diseases in the body will be able to treat genetic diseases fundamentally; carry out precise genetic engineering on crops to increase their yield or res...

Claims

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