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Method for determining content of curculigoside in curculigo orchioides by ASE-HPLC method

A technology of curculigoside and curculigo, which is applied in the field of chemical detection, can solve the problems of complex operation process of pharmacopoeia and high requirements for operators' operation skills, and achieve the effects of good repeatability, increased diffusion, and reduced surface tension

Inactive Publication Date: 2017-07-04
WUZHOU INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The operation process of the method in the Pharmacopoeia is complicated and requires high skills of the operator

Method used

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  • Method for determining content of curculigoside in curculigo orchioides by ASE-HPLC method

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Experimental program
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Embodiment 1

[0028] 1. Instruments and reagents

[0029] 1.1 Instruments:

[0030] Electronic analytical balance (XA205DU), ASE350 fast solvent extractor (American DIONEX company), ThermoU3000UHPLC liquid chromatograph.

[0031] 1.2 Reagents:

[0032] Water: Class I water in accordance with GB / T 6682;

[0033] Acetonitrile (C 2 h 3 N): chromatographically pure;

[0034] Phosphoric acid (H 3 PO 4 ): analytically pure

[0035] 2. Method

[0036] 2.1 Preparation of reference solution:

[0037] Take an appropriate amount of curculigoside reference substance, weigh it accurately, add methanol to make a solution containing 70 μg per 1 ml, and obtain it.

[0038] 2.2 Preparation of the test solution:

[0039] 2.2.1 "Chinese Pharmacopoeia" 2015 Edition Part I Preparation method for the test product:

[0040] Take about 1g of the powder of this product (passed through a No. 3 sieve), accurately weighed, accurately add 50ml of methanol, weigh it, heat and reflux for 2 hours, take it out,...

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Abstract

The invention discloses a method for determining content of curculigoside in curculigo orchioides by an ASE-HPLC method, which belongs to the field of chemical detection. The method has the advantages of good extraction effect and high detection precision, the method employs an ASE method to use methanol for extracting curculigo orchioides powder, then a methanol extract is collected; a HPLC method is used for determining the curculigoside content in the methanol extract, wherein, an employed mobile phase is acetonitrile-0.1% phosphoric acid. The method can substitute a pharmacopeia method for determining the content and controlling the quality of curculigoside in curculigo orchioides.

Description

technical field [0001] The invention belongs to the field of chemical detection, in particular to an ASE-HPLC method for determining the content of curculigoside in Curculigo curculigo. Background technique [0002] In 2015, the Pharmacopoeia recorded that about 1g of the powder of this product (passed through a No. 3 sieve) was taken, accurately weighed, 50ml of methanol was added accurately, weighed, heated to reflux for 2 hours, taken out, allowed to cool, weighed again, and supplemented with methanol. Lost weight, shake well, filter. Precisely measure 20ml of the follow-up filtrate, evaporate to dryness, add methanol to dissolve the residue, transfer to a 10ml measuring bottle, add methanol to the mark, shake well, filter, take the follow-up filtrate, and obtain. [0003] The detection conditions of liquid chromatography are as follows: octadecylsilane bonded silica gel is used as filler; acetonitrile-0.1% phosphoric acid solution (21:79) is used as mobile phase; detect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 王丽丽廖强欧妮韦日伟陈学松
Owner WUZHOU INST FOR FOOD & DRUG CONTROL